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Your search keyword '"Tang, Ming-Liang"' showing total 3 results
Start Over Author "Tang, Ming-Liang" Journal proceedings of the national academy of sciences of the united states of america
3 results on '"Tang, Ming-Liang"'

2. Telomere- and telomerase-interacting protein that unfolds telomere G-quadruplex and promotes telomere extension in mammalian cells.

Author

Feng Wang, Tang, Ming-liang, Zeng, Zhi-xiong, Wu, Ren-yi, Yong Xue, Hao, Yu-hua, Pang, Dai-Wen, Yong Zhao, and Zheng Tan

Subjects
*TELOMERES, *TELOMERASE, *CHROMOSOMES, *MAMMALS, *GENES
Abstract

Telomere extension by telomerase is essential for chromosome stability and cell vitality. Here, we report the identification of a splice variant of mammalian heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2), hnRNP A2", which binds telomeric DNA and telomerase in vitro. hnRNP A2" colocalizes with telomerase in Cajal bodies and at telomeres. In vitro assays show that hnRNP A2" actively unfolds telomeric G-quadruplex DNA, exposes 5 nt of the 3' telomere tail and substantially enhances the catalytic activity and processivity of telomerase. The expression level of hnRNP A2" in tissues positively correlates with telomerase activity, and overexpression of hnRNP A2" leads to telomere elongation in vivo. Thus, hnRNP A2" plays a positive role in unfolding telomere G-quadruplexes and in enhancing telomere extension by telomerase. [ABSTRACT FROM AUTHOR]

Published
2012
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3. Telomere- and telomerase-interacting protein that unfolds telomere G-quadruplex and promotes telomere extension in mammalian cells.

Author

Wang F, Tang ML, Zeng ZX, Wu RY, Xue Y, Hao YH, Pang DW, Zhao Y, and Tan Z

Subjects
Alternative Splicing, Animals, Base Sequence, Binding Sites genetics, Cell Line, DNA, Complementary chemistry, DNA, Complementary genetics, DNA, Complementary metabolism, HeLa Cells, Heterogeneous-Nuclear Ribonucleoprotein Group A-B chemistry, Heterogeneous-Nuclear Ribonucleoprotein Group A-B genetics, Humans, Liver metabolism, Male, Mice, Models, Biological, Nucleic Acid Conformation, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Small Interfering genetics, Rats, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Telomerase antagonists & inhibitors, Telomerase genetics, G-Quadruplexes, Heterogeneous-Nuclear Ribonucleoprotein Group A-B metabolism, Telomerase metabolism, Telomere metabolism, Telomere Homeostasis physiology
Abstract

Telomere extension by telomerase is essential for chromosome stability and cell vitality. Here, we report the identification of a splice variant of mammalian heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2), hnRNP A2*, which binds telomeric DNA and telomerase in vitro. hnRNP A2* colocalizes with telomerase in Cajal bodies and at telomeres. In vitro assays show that hnRNP A2* actively unfolds telomeric G-quadruplex DNA, exposes 5 nt of the 3' telomere tail and substantially enhances the catalytic activity and processivity of telomerase. The expression level of hnRNP A2* in tissues positively correlates with telomerase activity, and overexpression of hnRNP A2* leads to telomere elongation in vivo. Thus, hnRNP A2* plays a positive role in unfolding telomere G-quadruplexes and in enhancing telomere extension by telomerase.

Published
2012
Full Text
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