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To assess the clinical value of two-dimensional specking-tracking imaging (2D-STI) in the diagnosis of left atrial and left ventricular function in patients with coronary atherosclerotic disease (CAD). Methods: A total of 30 patients with CAD. who were admitted to Armed Police Corps Hospital of Hubei from December 2015 to December 2016. were chosen as experimental group: another 30 healthy subjects were selected as control group. Color Doppler ultrasound 2D-STI was used to measure the longitudinal strain of left ventricular segmental subendocardial myocardium, midmyocardium and subepicardial myocardium (TLSendo; TLSmid; TLSepi). the global longitudinal strain of left ventricular segmental subendocardial myocardium, midmyocardium and subepicardial myocardium (GLSendo; GLSmid: GLSepi). left ventricular systolic left atrial strain rate (SRs). left ventricular early diastolic left atrial peak strain rate (SRe) and left ventricular late diastolic left atrial strain rate (SRa). and the mean values were calculated (mSRs: mSRe; mSRa). The data were compared between the two groups. Results: The TLSendo [(21.45± 9.02)%] and TLSepi [(15.22± 5.12)%] of the experimental group were lower than those[(26.55± 7.59)% and (18.79± 3.77)% ] of the control group, the differences were statistically significant (P<0.05). The GLSendo [(21.07 ± 3.12)%]. GLSmid [(1S.11± 2.57)%] and GLSepi[( 15.79± 3.11)%] of the experimental group were lower than those[(23.55± 2.59)%, (20.82± 2.04)%. and (18.07± 2.00)%] of the control group,the differences were statistically significant (P<0.05). The mSRs(3.41± 0.68) and mSRe(-3.09± 0.82) of the experimental group were lower than those[(4.55± 0.69) and (-3. 67± 1.30)] of the control group: the mSRa (-4.47± 1.33) of the experimental group was higher than that [(-3.52± 1.44)] of the control group, the differences were statistically significant (P<0.05). The correlation coefficient between the two measurements of the same examiner was r=0.935(P<0.05), the correlation coefficient of the different examiners measurement results was r=0.931(P<0.05). Conclusion: 2D-STI can be used to quantitatively evaluate left ventricular function in patients with CAD. The main manifestations are the decrease of myocardial contractile function and the reduction of the left atrial reserve function in the left ventricle. [ABSTRACT FROM AUTHOR]