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Objective: To study the relationship between the expression of micro-93-5p (miR-93-5p) and microRNA-98-5p (miR-98-5p) in papillary thyroid carcinoma (PTC) and clinicopathological features, proliferation and invasion gene expression. Methods: 153 PTC patients who were underwent surgical resection in Guangdong Provincial Hospital of Traditional Chinese Medicine from October 2020 to October 2023 were selected as the research objects, and the intraoperatively resected cancer tissues and adjacent tissues were collected. The expression levels of miR-93-5p, miR-98-5p, proliferation gene and invasion gene mRNA in cancer tissues and adjacent tissues were detected and compared, and the relationship between the expression of miR-93-5p and miR-98-5p and the clinicopathological features of PTC patients were analyzed. The correlation between the expression levels of miR-93-5p and miR-98-5p and the mRNA expression of proliferation genes and invasion genes were analyzed by Pearson method. Results: The expression level of miR-93-5p in cancer tissues was higher than that in adjacent tissues, and the expression level of miR-98-5p was lower than that in adjacent tissues (P<0.05). The proportion of TNM stage III-IV, lymph node metastasis and poor differentiation in PTC patients with high expression of miR-93-5p were higher than those in PTC patients with low expression of miR-93-5p (P<0.05). The proportion of TNM stage III-IV, lymph node metastasis and poor differentiation in PTC patients with low expression of miR-98-5p were higher than those in PTC patients with high expression of miR-98-5p (P<0.05). The levels of proliferation gene programmed cell death factor 4 (PDCD4) and protein phosphatase 4 regulatory subunit (PP4R1) in cancer tissues were lower than those in adjacent tissues (P<0.05), and the levels of invasion gene metalloprotease disintegrin 9(ADAM9) and Bcl-6 co-inhibitor-like protein (BCORL1) were higher than those in adjacent tissues (P<0.05). Pearson analysis showed that, the expression of miR-93-5p was negatively correlated with the expression levels of proliferation genes PDCD4 and PP4R1, and positively correlated with the expression levels of invasion genes ADAM9 and BCORLI. The expression level of miR-98-5p was positively correlated with the levels of proliferation genes PDCD4 and PP4R1, and negatively correlated with the expression levels of invasion genes ADAM9 and BCORL1. Conclusion: The expression of miR-93-5p in cancer tissues of PTC patients is increase, and the expression of miR-98-5p is decrease, which are relate to clinicopathological features such as TNM stage, lymph node metastasis and differentiation degree, and can also promote the proliferation and invasion of PTC cancer cells. [ABSTRACT FROM AUTHOR]

Objective: To study the expression of Ribosomal protein L6 (RPL6) in the tissues of patients with prostate cancer.and to evaluate the role of the protein in the grade malignancy. Methods: A total of 117 patients with prostate disease, who were treated in First People's Hospital of Guangyuan from December 2013 to December 2015, were selected and divided into prostatitis group (n=63) and prostate cancer group(n=54) according to pathological results; in addition. 80 healthy people who underwent physical examination at the same time were enrolled as normal control group. The expression of RPL6 in the prostate tissue of three groups was detected by Western-blot. Serum tumor markers were detected by Enzyme-linked immunosorbent assay (ELISA). The levels of mKNA expression of proliferation gene and invasion gene were tested by RT-PCR. The relationship between RPL6 expression and malignant degree was analyzed by Pearson test. Results: The levels of RPL6 expression of prostate tissues in the prostate cancer group were higher than those in the prostatitis group and normal control group (P<0.05). The serum contents of prostate specific antigen (PSA), prostatic acid phosphatase (PSAP). urokinase-type plasminogen activator (uPA), and thioredoxin (TRX) in the prostate cancer group were higher than those in the prostatitis group and normal control group (P<0.05). The proliferation genes in the prostate tissues such as URG11 and PTEN mRNA in the prostate cancer group were higher than those in the prostatitis group and normal control group: PRDM5 mRNA expression in the prostate cancer group was lower than that in the prostatitis group and normal control group: the levels of invasion gene expression such as IgGHGl. TMPRSS2-ER. and PIK3C2B mRNA in the prostate cancer group were higher than those in the prostatitis group and normal control group (P<0.05). RPL6 expression in the tissues of prostate cancer patients was directly related to the content of tumor markers, proliferation and invasion genes (P<0.05). Conclusion: High expression of RPL6 is a reliable index for early diagnosis of prostate cancer, and it is directly related to the degree of malignancy, which is expected to be an important tool for the diagnosis and prognosis of prostate cancer. [ABSTRACT FROM AUTHOR]