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Objective: To explore the detection effect and accuracy of scattering immunoassay and fluorescence immunochromatography for detection of serum β2-microglobulin. Methods: 88 patients with liver and kidney function injury admitted to our hospital from October 2016 to August 2017 were selected in this study. And the level of serum β2-microglobulin was detected by scattering immunoassay and fluorescence immunochromatography, respectively. The detection results, sensitivity, specificity and accuracy of serum mirncroglobulin were compared between the two methods Results: The detection rate by fluorescence immunochromatography was significantly higher than that of scattering immunoassay, while the missed detection rate was significantly lower than that of the scattering immune method (P<0.05). The sensitivity, specificity and diagnostic coincidence rate of fluorescence immunotomography were significantly higher than those of scattering immunotomography (P<0.05). The levels of serum β2-microglobulin, creatinine, uric acid and urea of these patients were significantly higher than normal (P<0.05). In terms of accuracy, the accuracy of fluorescence immunochromatography between and within batches was significantly higher than that of scattering immunoassay (P<0.05). Conclusion: The application of fluorescence immunochromatography for detection of serum β2-microglobulin in patients has a higher detection coincidence rate, sensitivity and accuracy, which can meet the requirements of clinical detection process, and is beneficial to the diagnosis and treatment of patients. Therefore, fluorescence immunochromatography should be further popularized in clinical application. [ABSTRACT FROM AUTHOR]