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Objective: To investigate the effects of different acupuncture on the content of glial cell derived neurotrophic factor (GDNF) in substantia nigra, stomach and colon of rats with Parkinson's gastrointestinal dysfunction. Methods: The gastrointestinal dysfunction model of PD was established by injecting rotenone solution and detecting with apmorphine induction test. According to random number table method, 60 rats were divided into 6 groups: blank group, sham-operated group, model group, western medicine group, Tiaoshen Changzhi group and ordinary acupuncture group. The rats were treated for 2 weeks. The changes of body weight were observed, fecal moisture content, gastric content residual rate and intestinal propelling rate were detected, and GDNF content in substantia nigra, stomach and colon were detected by immunohistochemistry and ELISA. Results: Body weight and fecal water content: the western medicine group and the two acupuncture groups were significantly increased compared with the model group (P<0.01). The improvement degree in the western medicine group was better than that in the Tiaoshen Changzhi group (P<0.05), and the improvement degree in the Tiaoshen Changzhi group was better than in the ordinary acupuncture group (P<0.05). In terms of intestinal propulsion rate and gastric contents residual rate, there were significant differences between the western medicine group and the two acupuncture groups and model group (P<0.01). The improvement of the western medicine group was better than that of Tiaoshen Changzhi group (P<0.05), the Tiaoshen Changzhi group was better than the ordinary group (P<0.05). The changes of GDNF in substantia nigra, stomach and colon of midbrain were as follows: the contents of GDNF in blank group and sham group were higher than the model group (P<0.01). Compared with the model group, the content of the western medicine group and the two acupuncture groups increased (P<0.01). The efficacy of the western medicine group was better than that of the Tiaoshen Changzhi group (P<0.05), and the Tiaoshen Changzhi group was better than that of the conventional acupuncture group (P<0.05). Conclusion: Acupuncture treatment increased the content of GDNF in substantia nigra, stomach and colon of rats with PD gastrointestinal dysfunction, and the Tiaoshen Changzhi group was better than the conventional group and close to the western medicine group. One of the mechanisms of Tiaoshen Changzhi acupuncture in the treatment of gastrointestinal dysfunction of PD may be due to the enhancement of GDNF in the brain, stomach and colon. [ABSTRACT FROM AUTHOR]