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To investigate the expression of microRNA (miR)-1290 and mir-1307 in plasma exosomes of patients with ovarian cancer, and to analyze their relationship with clinicopathological parameters and diagnostic value. 60 patients with ovarian cancer who were diagnosed and treated in Jingjiang People's Hospital from January 2019 to January 2021 were selected as the ovarian cancer group, 50 female health examiners during the same period as the healthy control group and 52 patients with benign ovarian tumors as the benign tumor group. Real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-1290 and miR-1307 in plasma exosomes of each group. The relationship between miR-1290, miR-1307 and clinicopathological characteristics of patients with ovarian cancer were analyzed. The receiver operating characteristic curve(ROC)was used to analyze the diagnostic efficacy of plasma exosomes miR-1290 and miR-1307 for ovarian cancer. Compared with benign ovarian tumor group and healthy control group, the expression of plasma exosomes miR-1290 and miR-1307 in ovarian cancer group were higher (P＜0.05). The expression of miR-1290 in the ovarian cancer group was related to the FIGO stage, degree of differentiation and lymph node metastasis, and the level of miR-1307 was related to the FIGO stage and lymph node metastasis (P＜0.05). There was a significant positive correlation between the expression of plasma exosomes miR-1307 and miR-1290 of patients with ovarian cancer (r＝0.478, P＜0.05). The area under curve (AUC) (0.95CI) of plasma exosomes miR-1290, miR-1307 and combined application in the diagnosis of ovarian cancer were 0.726 (0.526 ~ 0.926), 0.724 (0.466 ~ 0.987) and 0.845 (0.750 ~ 0.933), respectively. The diagnostic efficiency of combined application was higher than that of single application. The expression levels of plasma exosomes miR-1290 and miR-1307 in patients with ovarian cancer are increased. The combined detection of miR-1290 and miR-1307 has high value in the early diagnosis of ovarian cancer, and is expected to become a new molecular marker of ovarian cancer. [ABSTRACT FROM AUTHOR]

Objective: To explore the role of miR-1290 in epithelial-mesenchymal transition of cervical cancer Hela cell. Methods: The radioresistant cervical cancer Hela cells and Siha cells were established by applying fractionated radiation consisted of a fraction dose of around 2 Gy of μ-ray. The differential expression patterns of miRNAs between radioresistant cells and parental cells were evaluated using miRNA microarray. The expression level of miR-1290 was detected in Hela cells treated under different conditions of hypoxia and radiation. The lentivirus of miR-1290 and miR-1290-inhibition were used to modulate the expression of miR-1290 in Hela cells. The wound healing and Transwell were used to compare the abilities of migration and invasion of Hela cells with different expression level of miR-1290. Western Blot was used to detect the expression level of E-cadherin and N-cadherin. Results: MiR-1290 expression were significantly upregulated in radioresistant cervical cancer cells (P<0.05). The radiation and hypoxia induced the expression of miR-1290 in cervical cancer Hela cells (P<0.05). Up-regulating the expression of miR-1290 enhanced abilities of migration and invasion of Hela cells and Hela cells presented the mesenchymal cell phenotypes (P<0.05). The miR-1290 overexpression decreased the expression of E-cadherin and increased the expression of N-cadherin in Hela cells (P<0.05). Conclusions: These results demonstrated that hypoxia and radiation up-regulated the expression of miR-1290 in cervical cancer Hela cells and miR-1290 promoted the EMT of Hela cells by modulating the expression of E-cadherin and N-cadherin. [ABSTRACT FROM AUTHOR]

Objective: To explore the effect of miR-1290 on cell proliferation, cell cycle and apoptosis on human umbilical ling-derived mesenchymal stem cell(MSCs). Methods: miR-1290 mimics was used to transfected into MSCs derived from human umbilical cord bling. The expression level of miR-1290 after transfection was tested by fluorimetric determination PCR. The proliferation ability was detected by MTT assay. Flow cytometry was applied to assess the cell cycle and apoptosis after transfection into MSCs.Results: RT-PCR results showed that the expression of miR-1290 was upregulated 12.2 fold after transfection. The MTT assay demonstrated that overexpression of miR-1290 could promote the proliferation of MSCs significantly. Compared with control and negative group(NC group), the period of G1 was decreased from(85.90±1.91)% to(76.35±2.03)%, while S and G2 period of cell cycle were much longer. The proliferation index increased to(23.75±3.01) compared wiith the control group(14.10±1.87). The result of apoptosis analyzed by flow cytometry showed that the rate of apoptosis of miR-1290(4.9±0.276)%was reduced compared with the negative control(8.2±0.891)%, P<0.05. Conclusions: miR-1290 could enhance the proliferation ability of MSCs, decrease the period of G1 and extend the S and G2 period, and repress the apoptosis of MSCs. [ABSTRACT FROM AUTHOR]