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Start Over Author "Huang, Yu‐Chieh" Topic aragonite Journal protein expression & purification

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1 results on '"Huang, Yu‐Chieh"'

1. Purification of recombinant nacre-associated mineralization protein AP7 fused with maltose-binding protein.

Author

Huang, Yu-Chieh, Chang, Hsun-Hui, Mou, Yun, Chi, Peter, Chan, Jerry Chun Chung, and Luo, Shih-Chi

Subjects
*BIOMINERALIZATION, *PROTEIN expression, *MALTOSE binding proteins, *ARAGONITE, *CALCIUM carbonate, *CD antigens, *CALCITE
Abstract

Highlights: [•] The first report of soluble bacterially expressed aragonite-associated protein. [•] MALDI-TOF detects more than one Ca2+ binding sites present within AP7. [•] CD suggests the recombinant AP7 is partially unstructured at neutral pH. [•] CaCO3 crystallization assay suggest AP7 can inhibit the crystal growth of calcite. [ABSTRACT FROM AUTHOR]

Published
2014
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