106 results on '"Li, Yifeng"'
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2. SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield
3. Acidic pH or salt treatment can convert soluble antibody aggregates in culture harvest into monomers and improve Protein A chromatography step yield
4. Antibody aggregation can lead to reduced Protein A binding capacity and low step yield
5. Complementary methods for monitoring hole-hole homodimer associated with a WuXiBody-based asymmetric bispecific antibody
6. Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: A case study
7. CaptureSelect FcXP affinity medium exhibits strong aggregate separation capability
8. Immunoglobulin-binding protein-based affinity chromatography in bispecific antibody purification: Functions beyond product capture
9. IgG-like bispecific antibody platforms with built-in purification-facilitating elements
10. SEC-HPLC analysis of column load and flow-through provides critical understanding of low protein a step yield
11. Monitoring of the disulfide scrambled species by mixed-mode SEC-HPLC during the purification of a bispecific antibody
12. Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case
13. Obtaining acidic and basic charge variants using a twin-column continuous chromatography system
14. CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation
15. Screening of six cation exchange resins for high binding capacity, monomer purity and step yield: A case study
16. Viral removal by column chromatography in downstream processing of monoclonal antibodies
17. Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact
18. Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody
19. Comparing the relative robustness of anion exchange and the corresponding mixed-mode chromatography on removing a weakly-bound byproduct: A case study of bispecific antibody purification
20. Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation
21. Evaluating a new mixed-mode resin Diamond MMC Mustang using Capto MMC ImpRes as a benchmark
22. Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody
23. Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody
24. Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography
25. Impact of salt concentration in mobile phase on antibody retention in Protein A, Protein L and KappaSelect affinity chromatography
26. Comparing the relative robustness of byproduct removal by wash and by elution in column chromatography for the purification of a bispecific antibody
27. Removing light chain-missing byproducts and aggregates by Capto MMC ImpRes mixed-mode chromatography during the purification of two WuXiBody-based bispecific antibodies
28. Protein L chromatography: A useful tool for monitoring/separating homodimers during the purification of IgG-like asymmetric bispecific antibodies
29. Removing a difficult-to-separate byproduct by Capto L affinity chromatography during the purification of a WuXiBody-based bispecific antibody
30. A potential downstream platform approach for WuXiBody-based IgG-like bispecific antibodies
31. Removing half antibody byproduct by Protein A chromatography during the purification of a bispecific antibody
32. Separating antibody species containing one and two kappa light chain constant region by KappaSelect affinity chromatography
33. Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography
34. Major cause of antibody artifact bands on non-reducing SDS-PAGE and methods for minimizing artifacts
35. Processing of high-salt-containing protein A eluate using mixed-mode chromatography in purifying an aggregation-prone antibody
36. Monitoring removal of hole-hole homodimer by analytical hydrophobic interaction chromatography in purifying a bispecific antibody
37. Sodium caprylate induced precipitation post Protein A chromatography as an effective means for host cell protein clearance
38. A method for improving protein A chromatography's aggregate removal capability
39. The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance
40. A brief introduction of IgG-like bispecific antibody purification: Methods for removing product-related impurities
41. A parallel demonstration of different resins' antibody aggregate removing capability by a case study
42. The application of caprylic acid in downstream processing of monoclonal antibodies
43. Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion proteins
44. Production of human antimicrobial peptide LL-37 in Escherichia coli using a thioredoxin–SUMO dual fusion system
45. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation
46. Erratum to “Recombinant production of antimicrobial peptides in Escherichia coli: A review” [Protein Express. Purif. 80 (2011) 206–267]
47. A novel protocol for the production of recombinant LL-37 expressed as a thioredoxin fusion protein
48. Recombinant production of antimicrobial peptides in Escherichia coli: A review
49. On-resin cleavage of bacterially expressed fusion proteins for purification of active recombinant peptides SK-29, KR-20, LL-29, and LL-23 from human sweat or skin
50. A novel method for purifying recombinant human host defense cathelicidin LL-37 by utilizing its inherent property of aggregation
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