1. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.
- Author
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Bastiaan-Net S, Pina-Pérez MC, Dekkers BJW, Westphal AH, America AHP, Ariëns RMC, de Jong NW, Wichers HJ, and Mes JJ
- Subjects
- Chromatography, Liquid, Tandem Mass Spectrometry, Allergens biosynthesis, Allergens chemistry, Allergens genetics, Anacardium chemistry, Anacardium genetics, Anacardium metabolism, Computer Simulation, Nuts chemistry, Nuts genetics, Nuts metabolism, Plant Proteins biosynthesis, Plant Proteins chemistry, Plant Proteins genetics, RNA-Seq
- Abstract
Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens., (© 2020 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society.)
- Published
- 2020
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