1. iSEE: Interface structure, evolution, and energy-based machine learning predictor of binding affinity changes upon mutations.
- Author
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Geng C, Vangone A, Folkers GE, Xue LC, and Bonvin AMJJ
- Subjects
- Binding, Competitive, Evolution, Molecular, Models, Molecular, Protein Binding, Protein Domains, Proteins chemistry, Proteins metabolism, Proto-Oncogene Proteins c-mdm2 chemistry, Proto-Oncogene Proteins c-mdm2 genetics, Proto-Oncogene Proteins c-mdm2 metabolism, Thermodynamics, Tumor Suppressor Protein p53 chemistry, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Computational Biology methods, Machine Learning, Mutation, Proteins genetics
- Abstract
Quantitative evaluation of binding affinity changes upon mutations is crucial for protein engineering and drug design. Machine learning-based methods are gaining increasing momentum in this field. Due to the limited number of experimental data, using a small number of sensitive predictive features is vital to the generalization and robustness of such machine learning methods. Here we introduce a fast and reliable predictor of binding affinity changes upon single point mutation, based on a random forest approach. Our method, iSEE, uses a limited number of interface Structure, Evolution, and Energy-based features for the prediction. iSEE achieves, using only 31 features, a high prediction performance with a Pearson correlation coefficient (PCC) of 0.80 and a root mean square error of 1.41 kcal/mol on a diverse training dataset consisting of 1102 mutations in 57 protein-protein complexes. It competes with existing state-of-the-art methods on two blind test datasets. Predictions for a new dataset of 487 mutations in 56 protein complexes from the recently published SKEMPI 2.0 database reveals that none of the current methods perform well (PCC < 0.42), although their combination does improve the predictions. Feature analysis for iSEE underlines the significance of evolutionary conservations for quantitative prediction of mutation effects. As an application example, we perform a full mutation scanning of the interface residues in the MDM2-p53 complex., (© 2018 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals, Inc.)
- Published
- 2019
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