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Your search keyword '"Dobos, Karen M."' showing total 9 results
Start Over Author "Dobos, Karen M." Journal proteomics
9 results on '"Dobos, Karen M."'

3. Deciphering the proteome of the in vivo diagnostic reagent “purified protein derivative” from Mycobacterium tuberculosis

Author

Cho, Yun Sang, primary, Dobos, Karen M., additional, Prenni, Jessica, additional, Yang, Hongliang, additional, Hess, Ann, additional, Rosenkrands, Ida, additional, Andersen, Peter, additional, Ryoo, Sung Weon, additional, Bai, Gill-Han, additional, Brennan, Michael J., additional, Izzo, Angelo, additional, Bielefeldt-Ohmann, Helle, additional, and Belisle, John T., additional

Published
2012
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6. Deciphering the proteomic profile of Mycobacterium leprae cell envelope

Author

Marques, Maria Angela M., primary, Neves-Ferreira, Ana Gisele C., additional, da Silveira, Erika K. Xavier, additional, Valente, Richard H., additional, Chapeaurouge, Alexander, additional, Perales, Jonas, additional, da Silva Bernardes, Renato, additional, Dobos, Karen M., additional, Spencer, John S., additional, Brennan, Patrick J., additional, and Pessolani, Maria Cristina V., additional

Published
2008
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7. Continued proteomic analysis ofMycobacterium leprae subcellular fractions

Author

Marques, Maria Angela M., primary, Espinosa, Benjamin J., additional, Xavier da Silveira, Erika K., additional, Pessolani, Maria Cristina V., additional, Chapeaurouge, Alex, additional, Perales, Jonas, additional, Dobos, Karen M., additional, Belisle, John T., additional, Spencer, John S., additional, and Brennan, Patrick J., additional

Published
2004
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9. Continued proteomic analysis of Mycobacterium leprae subcellular fractions.

Author

Marques MA, Espinosa BJ, Xavier da Silveira EK, Pessolani MC, Chapeaurouge A, Perales J, Dobos KM, Belisle JT, Spencer JS, and Brennan PJ

Subjects
Chromatography, Affinity, Cytosol metabolism, Electrophoresis, Gel, Two-Dimensional, Genome, Bacterial, Mass Spectrometry, Proteome, Spectrometry, Mass, Electrospray Ionization, Subcellular Fractions metabolism, Bacterial Proteins chemistry, Mycobacterium leprae metabolism, Proteomics methods
Abstract

Recently the sequence of the Mycobacterium leprae chromosome, the only known obligate intracellular mycobacterium, was completed. It has a dramatic reduction in functional genes, with a coding capacity of only 49.5%, the lowest one so far observed among bacterial genomes. The leprosy bacillus seems to preserve a minimal set of genes that allows its survival in the host. The identification of genes that are actually expressed by the bacterium is of high significance in the context of mycobacterial pathogenesis. In this current study, a proteomic approach was undertaken to identify the proteins present in the soluble/cytosol and membrane subcellular fractions obtained from armadillo derived M. leprae. Proteins from each fraction were separated by two-dimensional gel electrophoresis (2-DE) and identified by mass spectrometry. A total of 147 protein spots were identified from 2-DE patterns and shown to comprise products of 44 different genes, twenty eight of them corresponding to new proteins. Additionally, two highly basic proteins (with pI >10.0) were isolated by heparin affinity chromatography and identified by N-terminal sequencing. This study constitutes the first application of proteomics to a host-derived Mycobacterium.

Published
2004
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