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Role stress has been extensively studied in organizational psychology and the present study focused on two types of role stress: role conflict and role ambiguity. The purpose of the study is to examine the moderating effect of gender in the relationship between role stress and job satisfaction for software professionals which had received little attention in Indian context. A Structural Equation Modeling (SEM) is used to analyze the data which includes confirmatory factor analysis (CFA), path analysis and multi-group moderation analysis . The results of the study revealed that there was slight increase in negative effect of role conflict on job satisfaction and slight decrease in negative effect of role ambiguity on job satisfaction among female employees. In case of male there was no increase or decrease in negative effect of role conflict and role ambiguity on job satisfaction. This study found invariant moderation effect of gender on relationship between role stress (role conflict and role ambiguity) and job satisfaction. Research implications, suggestions for role stress management and scope for future research are provided. [ABSTRACT FROM AUTHOR]