Search

The dead time determination with the spreadsheet method has been conducted on the HPLC system with 2-alkanones homolog series, eluted with several composition ratios of acetonitrile/water solvent. The accuracy of the spreadsheet method is compared against the iteration method, according to the principle of (1) the smallest dead time; (2) the smallest standard deviation of the different dead time values given by the different solvent composition ratios; and (3) the highest linearity of the correlation between the natural logarithm of capacity factor ln(k') versus carbon number nc. The comparison results suggest that the spreadsheet method has equally good criteria with the iteration method. The use of acetonitrile/water solvent exhibits the improved accuracy of the dead time determination with smaller dead time values and standard deviation than the ones given by the methanol/water solvent. [ABSTRACT FROM AUTHOR]