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Start Over Author "Diana Gutsaeva" Journal redox biology
2 results on '"Diana Gutsaeva"'

1. Inhibiting microRNA-144 potentiates Nrf2-dependent antioxidant signaling in RPE and protects against oxidative stress-induced outer retinal degeneration

Author

Ravirajsinh N. Jadeja, Malita A. Jones, Ammar A. Abdelrahman, Folami L. Powell, Menaka C. Thounaojam, Diana Gutsaeva, Manuela Bartoli, and Pamela M. Martin

Subjects
Medicine (General), R5-920, Biology (General), QH301-705.5
Abstract

The retinal pigment epithelium (RPE) is consistently exposed to high levels of pro-oxidant and inflammatory stimuli. As such, under normal conditions the antioxidant machinery in the RPE cell is one of the most efficient in the entire body. However, antioxidant defense mechanisms are often impacted negatively by the process of aging and/or degenerative disease leaving RPE susceptible to damage which contributes to retinal dysfunction. Thus, understanding better the mechanisms governing antioxidant responses in RPE is critically important. Here, we evaluated the role of the redox sensitive microRNA miR-144 in regulation of antioxidant signaling in human and mouse RPE. In cultured human RPE, miR-144-3p and miR-144-5p expression was upregulated in response to pro-oxidant stimuli. Likewise, overexpression of miR-144-3p and -5p using targeted miR mimics was associated with reduced expression of Nrf2 and downstream antioxidant target genes (NQO1 and GCLC), reduced levels of glutathione and increased RPE cell death. Alternately, some protection was conferred against the above when miR-144-3p and miR-144-5p expression was suppressed using antagomirs. Expression analyses revealed a higher conservation of miR-144-3p expression across species and additionally, the presence of two potential Nrf2 binding sites in the 3p sequence compared to only one in the 5p sequence. Thus, we evaluated the impact of miR-144-3p expression in the retinas of mice in which a robust pro-oxidant environment was generated using sodium iodate (SI). Subretinal injection of miR-144-3p antagomir in SI mice preserved retinal integrity and function, decreased oxidative stress, limited apoptosis and enhanced antioxidant gene expression. Collectively, the present work establishes miR-144 as a potential target for preventing and treating degenerative retinal diseases in which oxidative stress is paramount and RPE is prominently affected (e.g., age-related macular degeneration and diabetic retinopathy). Keywords: Nrf2, Oxidative stress, microRNA, miR-144, Retinal pigment epithelium, RPE

Published
2020
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2. Inhibiting microRNA-144 potentiates Nrf2-dependent antioxidant signaling in RPE and protects against oxidative stress-induced outer retinal degeneration

Author

Manuela Bartoli, Ravirajsinh N. Jadeja, Pamela M. Martin, Folami L. Powell, Malita A Jones, Diana Gutsaeva, Ammar A. Abdelrahman, and Menaka Thounaojam

Subjects
Male, 0301 basic medicine, Retinal degeneration, Programmed cell death, NF-E2-Related Factor 2, Clinical Biochemistry, Retinal Pigment Epithelium, medicine.disease_cause, Models, Biological, Biochemistry, Nrf2, Antioxidants, Cell Line, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, microRNA, medicine, Animals, Humans, Antagomir, 3' Untranslated Regions, lcsh:QH301-705.5, lcsh:R5-920, Retinal pigment epithelium, Retinal Degeneration, Organic Chemistry, Retinal, medicine.disease, miR-144, 3. Good health, Cell biology, MicroRNAs, Oxidative Stress, 030104 developmental biology, medicine.anatomical_structure, GCLC, chemistry, lcsh:Biology (General), RNA Interference, RPE, sense organs, lcsh:Medicine (General), 030217 neurology & neurosurgery, Oxidative stress, Research Paper, Signal Transduction
Abstract

The retinal pigment epithelium (RPE) is consistently exposed to high levels of pro-oxidant and inflammatory stimuli. As such, under normal conditions the antioxidant machinery in the RPE cell is one of the most efficient in the entire body. However, antioxidant defense mechanisms are often impacted negatively by the process of aging and/or degenerative disease leaving RPE susceptible to damage which contributes to retinal dysfunction. Thus, understanding better the mechanisms governing antioxidant responses in RPE is critically important. Here, we evaluated the role of the redox sensitive microRNA miR-144 in regulation of antioxidant signaling in human and mouse RPE. In cultured human RPE, miR-144-3p and miR-144-5p expression was upregulated in response to pro-oxidant stimuli. Likewise, overexpression of miR-144-3p and -5p using targeted miR mimics was associated with reduced expression of Nrf2 and downstream antioxidant target genes (NQO1 and GCLC), reduced levels of glutathione and increased RPE cell death. Alternately, some protection was conferred against the above when miR-144-3p and miR-144-5p expression was suppressed using antagomirs. Expression analyses revealed a higher conservation of miR-144-3p expression across species and additionally, the presence of two potential Nrf2 binding sites in the 3p sequence compared to only one in the 5p sequence. Thus, we evaluated the impact of miR-144-3p expression in the retinas of mice in which a robust pro-oxidant environment was generated using sodium iodate (SI). Subretinal injection of miR-144-3p antagomir in SI mice preserved retinal integrity and function, decreased oxidative stress, limited apoptosis and enhanced antioxidant gene expression. Collectively, the present work establishes miR-144 as a potential target for preventing and treating degenerative retinal diseases in which oxidative stress is paramount and RPE is prominently affected (e.g., age-related macular degeneration and diabetic retinopathy)., Graphical abstract Image 1

Published
2020

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