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Tropospheric delay is a major error source that affects the performance of the Global Navigation Satellite Systems (GNSS) Real Time Kinematic (RTK) positioning especially for the medium/long-range baseline. Although high precision tropospheric delay can be estimated by GNSS carrier phase measurement, together with position and ambiguity, a relatively long period of convergence time is necessary. In this study, we develop a new GPS/BDS RTK algorithm constrained with a tropospheric delay parameters from Numerical weather prediction (NWP) model for medium/long-range baselines. The accuracy of the tropospheric delays derived from NWP is assessed through comparisons with the results of GAMIT (GNSS at MIT). The positioning performance with standard GPS RTK, standard GPS/BDS RTK, the developed NWP-constrained GPS RTK and NWP-constrained GPS/BDS RTK over medium/long-range baselines are compared in terms of the initialization time and the positioning accuracy. Experiment results show that the mean differences between the NWP and GAMIT zenith tropospheric delay (ZTD) are between −5.50 mm and 5.60 mm, and the RMS values of the NWP ZTD residuals are from 24.02 mm to 32.62 mm. A reduction in the initialization time of over 41% and 58% for medium- and long-range baselines can be achieved with the NWP-constrained RTK (both GPS alone and GPS/BDS RTK solutions) compared to the standard RTK solution, respectively. An improvement of over 30% can be found with the GPS/BDS RTK compared with that of the GPS alone RTK for both standard and the NWP-constrained modes. The positioning precision of NWP-constrained GPS/BDS RTK is better than 3 cm in the horizontal direction and better than 5 cm in the vertical direction, which satisfies the requirement of the precise positioning service. [ABSTRACT FROM AUTHOR]