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In dealing with the climate change externality of the fossil-fuel dominated transport sector, bio-fuels are widely seen as a solution. Through its Bioenergy Policy, Ghana seeks to improve oil supply security, save foreign exchange, create jobs and reduce emissions from the transport sector by integrating 20% biofuels into the transport fuel mix by 2030. This paper systematically analyses the transport fuel demand in Ghana to determine the biofuel supply target in 2020 and 2030 and evaluates the resource input requirements for integration of biofuels into the transport fuel mix. It provides a detailed picture of bio-fuel prospects in Ghana in the 2030 horizon. The research concludes that though significant yield improvement is required to meet the target, the target is achievable. [ABSTRACT FROM AUTHOR]