1. Knockdown of UCHL5IP causes abnormalities in γ-tubulin localisation, spindle organisation and chromosome alignment in mouse oocyte meiotic maturation.
- Author
-
Wang, Ya-Peng, Qi, Shu-Tao, Wei, Yanchang, Ge, Zhao-Jia, Chen, Lei, Hou, Yi, Ouyang, Ying-Chun, Schatten, Heide, Zhao, Jian-Guo, and Sun, Qing-Yuan
- Subjects
MICROTUBULES ,CHROMOSOMES ,MITOSIS ,MEIOSIS ,PROTEIN research - Abstract
UCHL5IP is one of the subunits of the haus complex, which is important for microtubule generation, spindle bipolarity and accurate chromosome segregation in Drosophila and human mitotic cells. In this study, the expression and localisation of UCHL5IP were explored, as well as its functions in mouse oocyte meiotic maturation. The results showed that the UCHL5IP protein level was consistent during oocyte maturation and it was localised to the meiotic spindle in MI and MII stages. Knockdown of UCHL5IP led to spindle defects, chromosome misalignment and disruption of γ-tubulin localisation in the spindle poles. These results suggest that UCHL5IP plays critical roles in spindle formation during mouse oocyte meiotic maturation. In mammals, meiosis is a necessary step to produce spermatozoa or oocytes for sexual reproduction, and γ-tubulin localises at the spindle poles to promote microtubule nucleation, which is critical for meiosis procession. In this research, we focussed on the protein UCHL5IP, one of the subunits of augmin, which is important for γ-tubulin localisation in mitosis, to explore its function in oocyte meiotic maturation. Knockdown of UCHL5IP disrupted γ-tubulin localisation and led to chromosome misalignment and spindle defects. Our research reveals the functions of UCHL5IP in mouse oocyte maturation and promotes understanding of the regulation of meiosis. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF