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Start Over Author "D, Royere" Journal reproduction nutrition development
2 results on '"D, Royere"'

1. Treatment of human spermatozoa with follicular fluid can influence lipid content and motility during in vitro capacitation

Author

D. Royere, S. Hamamah, C. Barthelemy, Jacques Lansac, M. A. Garrigue, JP Muh, M. Lanson, and Revues Inra, Import

Subjects
Male, medicine.medical_specialty, Motility, Biology, Incubation period, Membrane Lipids, chemistry.chemical_compound, Ovarian Follicle, Capacitation, Internal medicine, [SDV.BDD] Life Sciences [q-bio]/Development Biology, medicine, Humans, Incubation, Phospholipids, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, Cholesterol, Spermatozoa, Follicular fluid, In vitro, Body Fluids, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Endocrinology, chemistry, Sperm Motility, Female, Sperm Capacitation, Percoll
Abstract

In order to evaluate the role of human follicular fluid (HFF) on the fertilizing capacity of spermatozoa, we studied the effect of HFF on the lipid composition and on the movement characteristics of human spermatozoa. Spermatozoa (spz) from normospermic patients were prepared with a discontinuous Percoll gradient and incubated in Menezo B2 medium with or without a supplement of 20% HFF (HFF-Percoll spz and B2-Percoll spz respectively) for 2 and 24 h. After 2 h HFF incubation, percentage progressive motility, straight line velocity (VSL), and amplitude of lateral head displacement (ALH) were improved in HFF-Percoll spz as compared to B2-Percoll spz (P < or = 0.05). After a longer incubation period (24 h), lipid changes appeared in HFF-Percoll spz with lower levels of cholesterol (P = 0.02) and phospholipids (P = 0.05). No modification of the cholesterol/phospholipid ratio after 2 and 24 h of incubation in either B2-Percoll spz or HFF-Percoll spz was observed. Such decreases in lipid content of HFF-Percoll spz may be factors which could be taken into account as constituting part of membrane modifications during the capacitation process.

Published
1993
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2. Identification of hyaluronic acid and chondroitin sulfates in human follicular fluid and their effects on human sperm motility and the outcome of in vitro fertilization.

Author

Hamamah S, Wittemer C, Barthélemy C, Richet C, Zerimech F, Royere D, and Degand P

Subjects
Chondroitin Sulfates pharmacology, Female, Humans, Hyaluronic Acid pharmacology, Male, Chondroitin Sulfates analysis, Fertilization in Vitro, Follicular Fluid chemistry, Hyaluronic Acid analysis, Sperm Motility drug effects
Abstract

This study measured glycosaminoglycans (GAGs) in human follicular fluid (hFF) obtained from patients undergoing hormonal stimulation with combined GnRH agonist followed by gonadotropin hormone (hMG or FSH) for in vitro fertilization (IVF). The GAGs were partially characterized through the use of various mucopolysaccharidases and then their effects tested on human sperm motility. In hFF, the GAG and protein concentrations were 4.4 +/- 1.3 mg/L and 32.6 +/- 3.2 g/L respectively. Chondroitins (CS) and hyaluronic acid (HA) significantly stimulate sperm motion in comparison to the control. The oocytes inseminated with GAGs-pretreated spermatozoa showed a significantly higher rate of cleavage and pregnancy or delivered number. The present results suggest that hFF is a rich source of sulfated glycosaminoglycan (CS), and non-sulfated glycosaminoglycan (HA). The stimulatory effects of hFF on human sperm motility may well depend on CS and HA. GAGs pretreated sperm may enhance the fertilizing ability of spermatozoa and thus the IVF outcome.

Published
1996
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