1. Embryo Form Project: An original technique for the three-dimensional reconstruction of human embryo morphology
- Author
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Romain Cendre, Romain Tonnelet, Marc Braun, Vincent Delmas, Emilien Micard, Marc Labrousse, T. Naidich, Laboratoire d'anatomie et cytologie pathologiques [Reims], Centre Hospitalier Universitaire de Reims (CHU Reims), Centre d'Investigation Clinique - Innovation Technologique [Nancy] (CIC-IT), Centre d'investigation clinique [Nancy] (CIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Imagerie Adaptative Diagnostique et Interventionnelle (IADI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Unité de recherche en développement, imagerie et anatomie (URDIA - EA 4465), Université Paris Descartes - Paris 5 (UPD5), Mount Sinai School of Medicine, Department of Psychiatry-Icahn School of Medicine at Mount Sinai [New York] (MSSM), Service d’Anatomie Pathologique [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Centre d'Investigation Clinique - Innovation Technologique (CIC-IT), Icahn School of Medicine at Mount Sinai [New York] (MSSM)-Department of Psychiatry, and Université de Lorraine (UL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Gestational Age ,Morphology (biology) ,Three-dimensional reconstruction ,Biology ,Toxicology ,Imaging, Three-Dimensional ,Morphogenesis ,Humans ,Human embryo ,Human embryogenesis ,Dicom viewer ,DICOM viewer ,Staining and Labeling ,Orientation (computer vision) ,business.industry ,3D reconstruction ,Pattern recognition ,Embryo ,Microtomy ,Anatomy ,Embryo morphology ,Embryo, Mammalian ,Embryogenesis ,Artificial intelligence ,business ,Software ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Computer technology - Abstract
International audience; Our current knowledge on the developmental stages of human embryogenesis has derived from limited numbers of classical studies. Computer technology now permits accurate 3D reconstruction of embryo morphology from serial histological sections. We present a successful technique that uses either fresh or preserved serial histological sections to generate highly detailed 3D image reconstructions of very small human embryos. We present the data we obtained from the reconstruction in virtual space of previously sectioned 15 and 22.5mm embryos. Their morphologies were studied using a DICOM viewer which permitted the analysis of any specific structure in any required orientation. To our knowledge, this is the first time human embryos have been reconstructed in this way. We believe that this reconstruction technique could improve our knowledge on embryo morphogenesis, especially if coupled to the study of genes involved in embryonic development. It may also prove to be a useful pedagogical tool.
- Published
- 2015