1. First identification of Cryptosporidium parvum subtype IIaA20G1R1 in water buffalos (Bubalus bubalis)
- Author
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Thais Agostinho Martins, Sonália Ferreira da Paixão Guterres, Danilo Rodrigues Barros Brito, Mércia de Seixas, João Luis Garcia, Edjanio Gaspar Patrício, Felippe Danyel Cardoso Martins, Sérgio Tosi Cardim, and Priscilla Melo
- Subjects
0301 basic medicine ,Cryptosporidium parvum ,Veterinary medicine ,General Veterinary ,biology ,Buffaloes ,animal diseases ,Cattle Diseases ,Cryptosporidiosis ,Cryptosporidium ,030108 mycology & parasitology ,biology.organism_classification ,18S ribosomal RNA ,DNA sequencing ,03 medical and health sciences ,Feces ,parasitic diseases ,Animals ,Cattle ,Bubalus ,Restriction fragment length polymorphism ,Nested polymerase chain reaction - Abstract
Cryptosporidium can infect a wide variety of vertebrate animals, including mammals, birds, amphibians, reptiles, and fish. There are few molecular characterizations of Cryptosporidium isolated from water buffalo. Thus, the present study investigated the occurrence and molecular characterization of Cryptosporidium spp. in water buffalos by nested-PCR. Non-diarrheic feces were obtained from 122 water buffalo calves. All samples were tested by nested-PCR based on the 18S rRNA gene, after which positive samples were analyzed by RFLP and genetic sequencing. Sixteen fecal (13.1%) samples were positive, and RFLP showed that fifteen presented patterns consistent with C. ryanae and one with C. parvum. Sequencing of the gp60 gene from the C. parvum positive sample indicated the subtype IIaA20G1R1. This is the first identification of the IIaA20G1R1 subtype in water buffalos.
- Published
- 2017