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Avipattikara churna is an Ayurvedic medicine, it is a mixture of 14 herbal and mineral materials in powder form. It is used in Ayurvedic treatment of agnimandya (digestive impairment), malabandha (constipation), amlapitta (dyspepsia), arsa (haemorrhoids), mutrabandha (retention of urine), prameha (urinary disorders) and piles. Avipattikara churna is a totally polyherbal classical medicine without any side effects. In this study attempts to evaluate the main parameters of drug standardization and HPTLC finger print profile of avipattikara churna based on organoleptic evaluation, physical characteristics, physicochemical studies like pH, total ash, acid insoluble ash, loss on drying at 105°C, extractive values etc.and microbial load were carried out as per the API, IP, WHO and AOAC guidelines to substantiate the adulteration for quality control of herbal drugs. The set parameters were found to be sufficient to evaluate the churna formulation and can be used as reference standards for the quality control/quality assurance for Ayurvedic formulations.

The present study was carried out for evaluation of TLC fingerprint profiles of volatile oils/essential oils of the each ingredient of the polyherbal formulation of Trikatu churna and comparison of the volatile oil of the finished product or formulation as such. Essential oils/volatile oil contents are characteristic to the ingredients or raw botanicals to identify them easily. In the raw botanicals, where ever its presence is possible and its verification through the TLC/HPTLC fingerprint profile is the one of the best ways to identify and evaluate the quality of the finished formulation in the course of development of a standardization protocol as a quality control tool.