1. Differences in allergen-induced T cell activation between allergic asthma and rhinitis: Role of CD28, ICOS and CTLA-4
- Author
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Karine Botturi, Arnaud Cavaillès, Yannick Lacoeuille, Daniel Vervloet, Antoine Magnan, Institut du thorax, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pneumologie [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Service de Pneumologie [Grenoble], CHU Grenoble-Hôpital Michallon, and BMC, Ed.
- Subjects
Antigens, Differentiation, T-Lymphocyte ,Male ,MESH: Antigens, CD28 ,Allergy ,MESH: Asthma ,MESH: Flow Cytometry ,MESH: T-Lymphocyte Subsets ,Lymphocyte Activation ,medicine.disease_cause ,[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract ,T-Lymphocytes, Regulatory ,Allergen ,T-Lymphocyte Subsets ,Medicine ,CTLA-4 Antigen ,MESH: CTLA-4 Antigen ,IL-2 receptor ,MESH: Antigens, CD ,Cells, Cultured ,MESH: Cytokines ,MESH: Middle Aged ,biology ,FOXP3 ,CD28 ,hemic and immune systems ,Middle Aged ,Flow Cytometry ,MESH: Inducible T-Cell Co-Stimulator Protein ,medicine.anatomical_structure ,Cytokines ,Female ,MESH: Intradermal Tests ,MESH: Cells, Cultured ,Pulmonary and Respiratory Medicine ,Adult ,Rhinitis, Allergic, Perennial ,MESH: Hypersensitivity ,T cell ,chemical and pharmacologic phenomena ,Inducible T-Cell Co-Stimulator Protein ,Th2 Cells ,CD28 Antigens ,MESH: Th2 Cells ,Antigens, CD ,Hypersensitivity ,Humans ,Antigens, Dermatophagoides ,MESH: Lymphocyte Activation ,MESH: Antigens, Dermatophagoides ,Asthma ,lcsh:RC705-779 ,House dust mite ,MESH: Humans ,MESH: Rhinitis, Allergic, Perennial ,business.industry ,MESH: T-Lymphocytes, Regulatory ,Research ,MESH: Adult ,lcsh:Diseases of the respiratory system ,Intradermal Tests ,Th1 Cells ,biology.organism_classification ,medicine.disease ,MESH: Male ,respiratory tract diseases ,MESH: Th1 Cells ,MESH: Antigens, Differentiation, T-Lymphocyte ,Immunology ,[SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract ,business ,MESH: Female - Abstract
Background Th2 cell activation and T regulatory cell (Treg) deficiency are key features of allergy. This applies for asthma and rhinitis. However with a same atopic background, some patients will develop rhinitis and asthma, whereas others will display rhinitis only. Co-receptors are pivotal in determining the type of T cell activation, but their role in allergic asthma and rhinitis has not been explored. Our objective was to assess whether allergen-induced T cell activation differs from allergic rhinitis to allergic rhinitis with asthma, and explore the role of ICOS, CD28 and CTLA-4. Methods T cell co-receptor and cytokine expressions were assessed by flow cytometry in PBMC from 18 house dust mite (HDM) allergic rhinitics (R), 18 HDM allergic rhinitics and asthmatics (AR), 13 non allergic asthmatics (A) and 20 controls, with or without anti-co-receptors antibodies. Results In asthmatics (A+AR), a constitutive decrease of CTLA-4+ and of CD4+CD25+Foxp3+ cells was found, with an increase of IFN-γ+ cells. In allergic subjects (R + AR), allergen stimulation induced CD28 together with IL-4 and IL-13, and decreased the proportion of CTLA-4+, IL-10+ and CD4+CD25+Foxp3+ cells. Anti-ICOS and anti-CD28 antibodies blocked allergen-induced IL-4 and IL-13. IL-13 production also involved CTLA-4. Conclusions T cell activation differs between allergic rhinitis and asthma. In asthma, a constitutive, co-receptor independent, Th1 activation and Treg deficiency is found. In allergic rhinitis, an allergen-induced Treg cell deficiency is seen, as well as an ICOS-, CD28- and CTLA-4-dependent Th2 activation. Allergic asthmatics display both characteristics.
- Published
- 2011
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