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1. Detection of proline using a novel paper-based analytical device for on-site diagnosis of drought stress in plants

Author

Kyeong-Hwan Lee, Cheol Soo Kim, Young-Soo Choi, and Mi Rha Lee

Subjects
Paper, Drought stress, Proline, Arabidopsis, 01 natural sciences, 010305 fluids & plasmas, chemistry.chemical_compound, Stress, Physiological, Lab-On-A-Chip Devices, 0103 physical sciences, Instrumentation, 010302 applied physics, Detection limit, Dehydration, Ninhydrin, food and beverages, Equipment Design, Paper based, Microfluidic Analytical Techniques, Droughts, chemistry, Green color, Biological system, Biosensor
Abstract

We developed and characterized a paper-based microfluidic sensor for the on-site diagnosis of drought stress in plants. Proline was used as a biomarker for analyzing drought stress, which was extracted by a colorimetric method using the proline-ninhydrin reaction. Paper was used as the main sensor material for the on-site detection of proline as it is easily transportable and cost-effective. The paper-based sensor was fabricated using wax-printing and origami methods, and the sensor was precoated with ninhydrin to allow for easy and convenient on-site use. Furthermore, a sample-to-ninhydrin ratio of 1:2 was found to confer optimal sensitivity to the drought diagnosis sensor. The concentration of proline in a sample was quantified by red-green-blue analysis to determine the change in green color intensity levels in response to distinct proline concentrations, which were detected by the sensor. The limit of detection of proline using the devised sensor was 657 µM, and the green color intensity level decreased with increasing proline concentration. In addition, the sensor was validated in an experimental drought stress model with Arabidopsis and subjected to drought stress for 21 days, and the amount of proline detected was 10 mM. The devised paper-based microfluidic sensor highlights the possibility of the on-site evaluation of drought stress in plants with potential to be utilized in various agricultural areas in the future.

Published
2019

2. Design of a streaked radiography instrument for ICF ablator tuning measurements.

Author

Olson, R. E., Hicks, D. G., Spears, B. K., Celliers, P. M., Holder, J. P., Landen, O. L., Geissel, M., Kellogg, J. W., Bennett, G. R., Edens, A. D., Atherton, B. W., and Leeper, R. J.

Subjects
*LABORATORIES, *X-rays, *TECHNICAL specifications, *BUILDINGS, *PAPER
Abstract

A streaked radiography diagnostic has been proposed as a technique to determine the ablator mass remaining in an inertial confinement fusion ignition capsule at peak velocity. This instrument, the “HXRI-5,” has been designed to fit within a National Ignition Facility Diagnostic Instrument Manipulator. The HXRI-5 will be built at Sandia National Laboratories (SNL), and initial testing will be done at the SNL Z-Beamlet Facility. In this paper, we will describe the National Ignition Campaign requirements for this diagnostic, the instrument design, and the planned test experiments. [ABSTRACT FROM AUTHOR]

Published
2008
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3. Detection of proline using a novel paper-based analytical device for on-site diagnosis of drought stress in plants.

Author

Choi YS, Lee MR, Kim CS, and Lee KH

Subjects
Arabidopsis metabolism, Dehydration diagnosis, Dehydration metabolism, Equipment Design, Lab-On-A-Chip Devices, Microfluidic Analytical Techniques methods, Ninhydrin, Paper, Arabidopsis chemistry, Droughts, Microfluidic Analytical Techniques instrumentation, Proline analysis, Stress, Physiological physiology
Abstract

We developed and characterized a paper-based microfluidic sensor for the on-site diagnosis of drought stress in plants. Proline was used as a biomarker for analyzing drought stress, which was extracted by a colorimetric method using the proline-ninhydrin reaction. Paper was used as the main sensor material for the on-site detection of proline as it is easily transportable and cost-effective. The paper-based sensor was fabricated using wax-printing and origami methods, and the sensor was precoated with ninhydrin to allow for easy and convenient on-site use. Furthermore, a sample-to-ninhydrin ratio of 1:2 was found to confer optimal sensitivity to the drought diagnosis sensor. The concentration of proline in a sample was quantified by red-green-blue analysis to determine the change in green color intensity levels in response to distinct proline concentrations, which were detected by the sensor. The limit of detection of proline using the devised sensor was 657 µM, and the green color intensity level decreased with increasing proline concentration. In addition, the sensor was validated in an experimental drought stress model with Arabidopsis and subjected to drought stress for 21 days, and the amount of proline detected was 10 mM. The devised paper-based microfluidic sensor highlights the possibility of the on-site evaluation of drought stress in plants with potential to be utilized in various agricultural areas in the future.

Published
2019
Full Text
View/download PDF