1. [Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi]
- Author
-
E A, Amador, A, Acosta Domínguez, M E, Sarmiento, C, Hidalgo Grass, A, Fachado Carvajales, T, Vidal Martínez, J L, Leyva, and L, Fonte Galindo
- Subjects
Male ,Genomic Library ,Mice ,Mice, Inbred BALB C ,Immunoglobulin G ,Trypanosoma cruzi ,Animals ,Antibodies, Protozoan ,DNA, Protozoan - Abstract
A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi. more...
- Published
- 2000