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Introduction: The high demand for physical fitness in elite athletes together with the risk of exceeding training limits makes controlled adaptation to this necessary. It is reported that markers such as Creatine kinase (CPK), urea, cortisol and testosterone are parameters used in assessing physiological and metabolic stress in high-performance athletes in training. Objectives: To evaluate the initial behavior of biochemical markers (CPK, urea, testosterone and cortisol) in a group of high performance athletes from Valle del Cauca. Methodology: This is a descriptive study of 15 monofin athletes that was approved by the ethics committee of the Universidad del Valle. The training session in water varied in intensity and volume depending on the work plan for functional areas according to lactate levels, conditioning of the athlete, and the type of event involved. After two months of general training a blood sample was taken post-training for the 15 athletes to make a determination of the differing markers. Results: The value of the markers found did not exceed the reference values: CPK-women: 24-170 U/L, CPK- men: 24-190 U/L, UREA: 10-50mg/dl, CORTISOL: 525 ug/dl, TESTOSTERONE-men: 2.4 - 18.4 ng/ml, TESTOSTERONE-women: 0.0 - 0.81 ng/dl. Conclusion: The training of the athletes during the period studied did not exceed their adaptive capacity to physiological stress because the values found were below the reference values. [ABSTRACT FROM AUTHOR]