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Start Over Journal revista iberoamericana de micologia
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1. [Conservation of Malassezia strains in blotting paper]

Author

Laura, Ramos, Silvana, Ramadán, Clara, López, Lucía, Bulacio, and Soledad, Mellado

Subjects
Cryopreservation, Glycerol, Paper, Cryoprotective Agents, Malassezia, Phenotype, Genotype, Preservation, Biological, Mycology, Reference Standards
Abstract

Reference strains belonging to the genus Malassezia were analyzed to evaluate, by comparison, different preservation systems such us subculture, freezing at -80 degrees C in glycerol, and blotting paper-disc conservation. The viability, phenotypic and genotypic characteristics of the strains used in this study was evaluated. The blotting paper method was found to be advantageous to preserve Malassezia spp strains due to both, its simple implementation in the laboratory and its efficiency.

Published
2006

2. [Enzymatic activity and degradation of different kinds of organic wastes by Saccobolus saccoboloides (Fungi, Ascomycotina)]

Author

Luis Alberto, Diorio, Flavia, Forchiassin, Víctor Leandro, Papinutti, and Diana Viviana, Sueldo

Subjects
Paper, Glycoside Hydrolases, Industrial Waste, Lignin, Wood, Culture Media, Refuse Disposal, Fungal Proteins, Xylan Endo-1,3-beta-Xylosidase, Industrial Microbiology, Biodegradation, Environmental, Xylosidases, Ascomycota, Cellulase, Amylases, Cellulose
Abstract

The ability to degrade organic solid wastes by the fungus Saccobolus saccoboloides was studied. The organism, unusual in such studies, was cultivated in synthetic liquid media with agitation, and on day 8 of growth the mycelium was passed to flasks with trimming. On day 16 of growth, the trimming degradation was assesed by carboxymethylcellulase, xylanase, and amylase activities evaluation, and NaOH 1% hydrolysis. Later on, the type of waste was modified (trimming, filter paper, newspaper, cardboard, sawdust and wood shaving were used) as well as the mass (300-1800 mg/flask). In these cases the enzymatic activities increased between 300 and 600 mg/flask. The total separation of the cellular components in all types of paper and cardboard was observed, together with a high loss of weight. S. saccoboloides was not able to degrade the wood wastes

Published
2003

3. [Conservation of Malassezia strains in blotting paper].

Author

Ramos L, Ramadán S, López C, Bulacio L, and Mellado S

Subjects
Cryopreservation, Cryoprotective Agents pharmacology, Genotype, Glycerol, Mycology standards, Paper, Phenotype, Reference Standards, Malassezia drug effects, Malassezia genetics, Mycology methods, Preservation, Biological
Abstract

Reference strains belonging to the genus Malassezia were analyzed to evaluate, by comparison, different preservation systems such us subculture, freezing at -80 degrees C in glycerol, and blotting paper-disc conservation. The viability, phenotypic and genotypic characteristics of the strains used in this study was evaluated. The blotting paper method was found to be advantageous to preserve Malassezia spp strains due to both, its simple implementation in the laboratory and its efficiency.

Published
2006
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4. [Enzymatic activity and degradation of different kinds of organic wastes by Saccobolus saccoboloides (Fungi, Ascomycotina)].

Author

Diorio LA, Forchiassin F, Papinutti VL, and Sueldo DV

Subjects
Amylases metabolism, Ascomycota growth & development, Biodegradation, Environmental, Cellulose metabolism, Culture Media, Glycoside Hydrolases metabolism, Lignin metabolism, Wood, Xylan Endo-1,3-beta-Xylosidase, Xylosidases metabolism, Ascomycota enzymology, Cellulase, Fungal Proteins metabolism, Industrial Microbiology, Industrial Waste, Paper, Refuse Disposal methods
Abstract

The ability to degrade organic solid wastes by the fungus Saccobolus saccoboloides was studied. The organism, unusual in such studies, was cultivated in synthetic liquid media with agitation, and on day 8 of growth the mycelium was passed to flasks with trimming. On day 16 of growth, the trimming degradation was assesed by carboxymethylcellulase, xylanase, and amylase activities evaluation, and NaOH 1% hydrolysis. Later on, the type of waste was modified (trimming, filter paper, newspaper, cardboard, sawdust and wood shaving were used) as well as the mass (300-1800 mg/flask). In these cases the enzymatic activities increased between 300 and 600 mg/flask. The total separation of the cellular components in all types of paper and cardboard was observed, together with a high loss of weight. S. saccoboloides was not able to degrade the wood wastes

Published
2003