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Goal: The objective of our study was to assess the evolution of the knowledge and behavior of pharmacy pharmacists in the fight against malaria with a view to involving officer's pharmacists in the fight against malaria. Method: This was a cross-sectional descriptive study that took place in two phases in the private dispensaries of the Bamako district of Mali. The first phase of the study (initial investigation) took place from April to May 2014 and covered 55 pharmacists from communes III and VI in Bamako. The second phase (monitoring) took place from November 2014 to November 2015, after training of all dispensary pharmacists in the Bamako district in accordance with the PNLP guidelines, which concerned all pharmacists in the Bamako district. Result: This revealed that some indicators are significantly improved: possession of a national policy document; Knowledge of drugs recommended for simple malaria; Practice and sale of rapid diagnostic tests for malaria. Other indicators have increased: availability of ACTs; Sale of insecticide-treated mosquito nets; Behavior before a suspicion of simple malaria; Behavior before suspicion of severe malaria. Conclusion: Thus, an involvement of officer pharmacists in malaria epidemiological surveillance can significantly reduce malaria morbidity and mortality. We recommend to the pharmacists the dispensation of the antimalaria ones according to the recommendations of the national program of fight against the malaria.