1. In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster .
- Author
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Donelick HM, Talide L, Bellet M, Aruscavage PJ, Lauret E, Aguiar ERGR, Marques JT, Meignin C, and Bass BL
- Subjects
- Animals, DNA Helicases genetics, Drosophila Proteins genetics, Drosophila melanogaster enzymology, Drosophila melanogaster growth & development, Female, In Vitro Techniques, Male, MicroRNAs genetics, RNA Helicases genetics, RNA, Double-Stranded genetics, RNA, Small Interfering genetics, Ribonuclease III genetics, Adenosine Triphosphate metabolism, DNA Helicases metabolism, Drosophila Proteins metabolism, Drosophila melanogaster genetics, Mutation, RNA Helicases metabolism, RNA, Double-Stranded metabolism, Ribonuclease III metabolism
- Abstract
In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform•PAZ domain to initiate processing of dsRNA with 3' overhangs (ovrs). To understand the relationship of these in vitro observations to roles of Dcr-2 in vivo, we compared in vitro effects of two helicase mutations to their impact on production of endogenous and viral siRNAs in flies. Consistent with the importance of the helicase domain in processing BLT dsRNA, both point mutations eliminated processing of BLT, but not 3'ovr, dsRNA in vitro. However, the mutations had different effects in vivo. A point mutation in the Walker A motif of the Hel1 subdomain, G31R, largely eliminated production of siRNAs in vivo, while F225G, located in the Hel2 subdomain, showed reduced levels of endogenous siRNAs, but did not significantly affect virus-derived siRNAs. In vitro assays monitoring dsRNA cleavage, dsRNA binding, ATP hydrolysis, and binding of the accessory factor Loquacious-PD provided insight into the different effects of the mutations on processing of different sources of dsRNA in flies. Our in vitro studies suggest effects of the mutations in vivo relate to their effects on ATPase activity, dsRNA binding, and interactions with Loquacious-PD. Our studies emphasize the importance of future studies to characterize dsRNA termini as they exist in Drosophila and other animals., (© 2020 Donelick et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
- Published
- 2020
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