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Conventional tillage has been the most common agriculture practice in most areas of Eastern Danube Plain of Romania. This tillage system requires a high energy input and may cause water loss and also long-term soil physical degradation. The results of our research prove that adopting conservation tillage practices more or less similar winter wheat, maize and soybean yields with similar quality levels can be obtained. The aim of this study was to assess new or additional tillage methods, which are able to maintain high crop yields and good grain quality. For this purpose, three tillage treatments (P: conventional tillage; C: chisel plough tillage; NT: no tillage), with and without deep sub-soiling, were studied during eight years (2008-2015) at Fundulea, which is located in the middle of Eastern Romanian Danube Plain. Protein content, thousand kernel weight (TKW) and grain yield of winter wheat were significantly influenced by weather conditions and soil tillage treatments. Maize protein content and grain yield were significantly influenced by weather conditions, deep sub-soiling and also by soil tillage treatments. Soybean protein content, TKW, and grain yield were also significantly influenced by weather conditions and soil tillage treatments. [ABSTRACT FROM AUTHOR]