1. Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology
- Author
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Magnus T. Borgström, Ralf Fliegert, Gabriele Loers, Parisa Bayat, Andreas H. Guse, Cornelius Fendler, Irene Fernandez-Cuesta, Robert H. Blick, Robert Zierold, Björn Philipp Diercks, Gaute Otnes, Carsten Ronning, Jann Harberts, and Aune Koitmäe
- Subjects
Morphology (linguistics) ,Materials science ,General Chemical Engineering ,Nanowire ,Substrate (chemistry) ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Jurkat cells ,0104 chemical sciences ,Cell membrane ,medicine.anatomical_structure ,Cell culture ,medicine ,Biophysics ,Viability assay ,Patch clamp ,0210 nano-technology - Abstract
Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al2O3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.
- Published
- 2019
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