1. Determination of insecticides’ lethal concentrations and metabolic enzyme levels in Triatoma dimidiata
- Author
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Arturo Acero-Sandoval, Américo D. Rodríguez, Mario H. Rodriguez, Jose Genaro Ordonez-Gonzalez, Teresa López-Ordóñez, Francisco Solis-Santoyo, and R Patricia Penilla-Navarro
- Subjects
Nymph ,Veterinary medicine ,Insecticides ,triatoma dimidiata ,Population ,cytochromes p450 ,Propoxur ,World Health Organization ,Mixed Function Oxygenases ,Insecticide Resistance ,Lethal Dose 50 ,chemistry.chemical_compound ,esterases ,Cytochrome P-450 Enzyme System ,Nitriles ,Pyrethrins ,parasitic diseases ,ace¬tylcholinesterase ,Animals ,Triatoma dimidiata ,Triatoma ,education ,glutathione s-transferase ,Glutathione Transferase ,education.field_of_study ,biology ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Glutathione ,Monooxygenase ,biology.organism_classification ,Deltamethrin ,chemistry ,Acetylcholinesterase ,Malathion ,Feasibility Studies ,lethal concentrations - Abstract
The feasibility of the use of WHO impregnated paper and biochemical assays to determine lethal concentrations (LC50 and LC99) and insecticide metabolic enzyme levels of Triatoma dimidiata.LC50 and LC99 were calculated with WHO papers impregnated at different concentrations of malathion, propoxur and deltamethrin; the percentage of insensitive acetylcholinesterase (iAChE); and the levels of esterases, glutathione S-transferases, and monooxygenases in laboratory nymphs of the first stage (5 to 7 days), were undertaken using the WHO biochemical assays.Respectively the LC50 and LC99 μg/cm2 obtained for malathion were 43.83 and 114.38, propoxur 4.71 and 19.29, and deltamethrin 5.80 and 40.46. A 30% of the population had an iAChE, and only a few individuals had high P450 and β-eterase levels.Impregnated papers and biochemical tests developed by WHO for other insects, proved to be feasible methods in monitoring insecticide resistance and metabolic enzymes involved in T. dimidiata.La factibilidad de usar los papeles impregnados y ensayos bioquímicos según la OMS para determinar concentraciones letales (CL50 y CL99) y niveles enzimáticos en la resistencia a insecticidas en Triatoma dimidiata.Se calcularon la CL50 y CL99 con papeles impregnados según la OMS a diferentes concentraciones de malatión, propoxur y deltametrina; el porcentaje de acetilcolinesterasa insensible (iAChE); y los niveles de esterasas, glutatión S-transferasas, y monooxigenasas en ninfas de laboratorio del estadio I (5-7 días) se determinaron usando los ensayos bioquímicos según la OMS.Se obtuvieron las CL50 y CL99 μg / cm2 respectivamente para malatión 43.83 y 114.38, propoxur 4.71 y 19.29, y deltametrina 5.80 y 40.46. Un 30% de las chinches tuvo iAChE, y sólo pocos individuos tuvieron niveles superiores de P450 y β-eterasas.Los papeles impregnados y ensayos bioquímicos que describe la OMS para otros insectos demostraron ser métodos factibles para monitorear la resistencia a insecticidas y las enzimas metabólicas involucradas en T. dimidiata.
- Published
- 2020