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Your search keyword '"Jakob Stenman"' showing total 2 results
Start Over Author "Jakob Stenman" Journal scandinavian journal of clinical and laboratory investigation
2 results on '"Jakob Stenman"'

1. Tumor expression of human chorionic gonadotropin beta mRNA and prognosis of prostate cancer treated by radical prostatectomy

Author

Kristina Hotakainen, Ulf-Håkan Stenman, Antti Rannikko, Anna Lempiäinen, Tuomas Mirtti, Susanna Lintula, Jakob Stenman, Anna Bützow, Department of Clinical Chemistry and Hematology, University of Helsinki, Research Program in Systems Oncology, University Management, HUSLAB, Department of Pathology, Institute for Molecular Medicine Finland, Department of Surgery, Urologian yksikkö, HUS Abdominal Center, Staff Services, Medicum, and Päivi Hotakainen / Principal Investigator

Subjects
0301 basic medicine, Male, medicine.medical_treatment, Clinical Biochemistry, SERUM, Human chorionic gonadotropin, Prostate cancer, 0302 clinical medicine, Prostate, Medicine, Chorionic Gonadotropin, beta Subunit, Human, URINARY CELLS, TRANSCRIPTION, chorionic gonadotropin beta, reproductive and urinary physiology, prostate, biology, Prostatectomy, General Medicine, Middle Aged, Prognosis, medicine.anatomical_structure, 030220 oncology & carcinogenesis, embryonic structures, ATP synthase alpha/beta subunits, Human, endocrine system, Sensitivity and Specificity, 03 medical and health sciences, Cell Line, Tumor, expression, cancer, Humans, RNA, Messenger, Gene, Aged, Messenger RNA, business.industry, Prostatic Neoplasms, BLADDER, CELL CARCINOMA, medicine.disease, Survival Analysis, HCG-BETA, 030104 developmental biology, SUBUNIT GENES, TISSUE, MARKER, Chorionic gonadotropin beta, Cancer research, biology.protein, 3111 Biomedicine, business
Abstract

The beta subunit of human chorionic gonadotropin (hCG beta) is encoded by six genes (CGB) classified as type I and type II. CGB mRNA is produced in large amounts by trophoblastic tissues and in small amounts by several cancerous tissues including prostate cancer and by a few benign tissues, including the prostate. Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was used to study the expression levels of all CGB mRNAs together (total CGB mRNA) and the two types of CGB mRNA separately in non-cancerous (n = 74) and cancerous prostatic tissue obtained by radical prostatectomy (n = 193). RNA was isolated from formalin-fixed paraffin-embedded (FFPE) samples and mRNA levels of CGB were correlated with disease-specific survival. Total CGB mRNA concentrations were significantly lower (p

Published
2019

2. Quantification of prostate specific antigen mRNA levels in circulation after prostatic surgery and endocrine treatment by quantitative reverse transcription-polymerase chain reaction

Author

S. Vesalainen, Antti Rannikko, Ulf-Håkan Stenman, Susanna Lintula, W.‐M. Zhang, Patrik Finne, and Jakob Stenman

Subjects
Male, medicine.medical_specialty, medicine.medical_treatment, Clinical Biochemistry, Urology, Prostatic Hyperplasia, Cell Line, Prostate cancer, Biopsy, medicine, Humans, Orchiectomy, RNA, Messenger, Transurethral resection of the prostate, Prostatectomy, medicine.diagnostic_test, business.industry, Reverse Transcriptase Polymerase Chain Reaction, Transurethral Resection of Prostate, General Medicine, Prostate-Specific Antigen, medicine.disease, Reverse transcription polymerase chain reaction, Prostate-specific antigen, Real-time polymerase chain reaction, Female, business
Abstract

Various methods to detect prostatic cells in circulation have given conflicting results. This is probably because qualitative rather than quantitative methods have been used to detect mRNA from prostatic cells. A quantitative method has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) for detection of prostate specific antigen (PSA) mRNA in peripheral blood. A competitive internal mRNA standard was used for quantification of absolute amounts of PSA mRNA. The detection limit of the assay was 7 copies of mRNA, and the highest level of circulating PSA mRNA in 88 control subjects was 25 copies per milliliter of blood. This method was used to study the influence of prostatic surgery and endocrine treatment on prostatic cells in the circulation of 56 patients undergoing biopsy, radical prostatectomy, transurethral resection of the prostate (TURP), orchiectomy, or androgen blockade. Blood samples were drawn before, during and up to 26 weeks after these procedures had been carried out. The highest level of PSA mRNA in controls was 25 copies per milliliter of blood. After RP, TURP or orchiectomy, PSA mRNA levels increased above this level in 27%, 29%, and 25% of the samples, respectively. After prostate biopsy, two out of 15 patients became positive. PSA mRNA levels that were elevated by surgery became undetectable within 1-3 days. No significant correlation was found between PCR positivity and the clinical characteristics of the patients. It is concluded that the level of PSA mRNA in peripheral blood increases after prostatic surgery, indicating temporary dissemination of prostatic cells. However, preoperative levels do not correlate with serum PSA, stage or grade.

Published
2004

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