Search

Variation in the expression of blood group antigen A in different areas of the human oral mucous membrane was investigated using a double layer immunofluorescence staining technique. The expression of the antigen was semiquantitated by a serial two-fold dilution titration. Comparison between cheek, commissure, lower lip, and hard palate was made. It was not possible to demonstrate any difference in expression of blood group antigen between cheek, commissure, and lower lip. However, in most cases a marked difference in blood group antigen A was found between cheek and hard palate, the latter demonstrating a much weaker expression. [ABSTRACT FROM AUTHOR]

All normal human sera examined have been shown to contain complement-fixing antibody to an antigen present in stratum corneum of human skin. By immune adherence with human sera and sections of frozen biopsy specimens, the antigen was found to be present in sections of keratotic and parakeratotic areas of human gingiva, tongue, alveolar, buccal, and palatal mucosa. Serum from biopsy donors mediated the adherence reaction. Absorption with a phenol-water extract (water phase) of stratum corneum of skin removed the antibodies. The antigen was not detected in non-keratinized areas of human oral mucosa, including gingival crevice and cuff epithelium. Erythrocytes also adhered to keratinized epithelium in gingival biopsy specimens fixed in ethanol and cleared in xylene at 4° C prior to sectioning and incubation with human serum. [ABSTRACT FROM AUTHOR]