1. Organization of the Mitotic Chromosome.
- Author
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Naumova, Natalia, Imakaev, Maxim, Fudenberg, Geoffrey, Ye Zhan, Lajoie, Bryan R., Mirny, Leonid A., and Dekker, Job
- Subjects
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CELL cycle , *CHROMOSOMES , *MOLECULAR structure , *MOLECULAR recognition , *MITOSIS , *MATHEMATICAL models , *CHROMATIN - Abstract
Job Dekker1 Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two distinct three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell type-specific organization described previously for nonsynchronous cells is restricted to interphase. In metaphase, we identified a homogenous folding state that is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we found that metaphase Hi-C data are inconsistent with classic hierarchical models and are instead best described by a linearly organized longitudinally compressed array of consecutive chromatin loops. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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