1. Resolution of Sister Telomere Association Is Required for Progression Through Mitosis
- Author
-
Susan Smith and Jasmin N. Dynek
- Subjects
DNA Replication ,Mitosis ,Chromatids ,Biology ,Transfection ,Anaphase-Promoting Complex-Cyclosome ,Chromosome Segregation ,Centromere ,Humans ,Sister chromatids ,RNA, Small Interfering ,In Situ Hybridization, Fluorescence ,Metaphase ,Anaphase ,Genetics ,Tankyrases ,Multidisciplinary ,Cohesin ,Ubiquitin-Protein Ligase Complexes ,Chromosome ,Telomere ,Establishment of sister chromatid cohesion ,Phenotype ,biological phenomena, cell phenomena, and immunity ,HeLa Cells - Abstract
Cohesins keep sister chromatids associated from the time of their replication in S phase until the onset of anaphase. In vertebrate cells, two distinct pathways dissociate cohesins, one acts on chromosome arms and the other on centromeres. Here, we describe a third pathway that acts on telomeres. Knockdown of tankyrase 1, a telomeric poly(ADP-ribose) polymerase caused mitotic arrest. Chromosomes aligned normally on the metaphase plate but were unable to segregate. Sister chromatids separated at centromeres and arms but remained associated at telomeres, apparently through proteinaceous bridges. Thus, telomeres may require a unique tankyrase 1–dependent mechanism for sister chromatid resolution before anaphase.
- Published
- 2004
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