1. Optimization of Virulence Functions Through Glucosylation of Shigella LPS
- Author
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Muriel Delepierre, Nicholas P. West, Philippe J. Sansonetti, Joëlle Mounier, Stéphanie Guadagnini, Ada Prochnicka-Chalufour, Christoph M. Tang, Rachel M. Exley, Myriam Tanguy, Claude Parsot, Marie-Christine Prévost, Centre for Molecular Microbiology and Infection, Faculty of Medicine-Department of Infectious Diseases, Pathogénie Microbienne Moléculaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Microscopie électronique (Plate-forme), Institut Pasteur [Paris], Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Operon ,MESH: Mutation ,MESH: Microscopy, Electron, Scanning ,MESH: Shigella flexneri ,MESH: Dysentery, Bacillary ,Lipopolysaccharide ,MESH: Rabbits ,Virulence ,MESH: Neutrophils ,MESH: Virulence ,medicine.disease_cause ,Type three secretion system ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Antigen ,medicine ,MESH: Animals ,Shigella ,Secretion ,MESH: Bacterial Adhesion ,MESH: Bacteriophages ,MESH: Carbohydrate Conformation ,MESH: O Antigens ,030304 developmental biology ,MESH: Hydrophobicity ,0303 health sciences ,Multidisciplinary ,Innate immune system ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,biology ,030306 microbiology ,MESH: Serotyping ,MESH: Glycosylation ,biology.organism_classification ,Enterobacteriaceae ,Virology ,MESH: Glucose ,chemistry ,MESH: Microscopy, Electron, Transmission ,MESH: Intestinal Mucosa ,MESH: Immunity, Innate ,MESH: Lipopolysaccharides - Abstract
Shigella , the leading cause of bacillary dysentery, uses a type III secretion system (TTSS) to inject proteins into human cells, leading to bacterial invasion and a vigorous inflammatory response. The bacterium is protected against the response by the O antigen of lipopolysaccharide (LPS) on its surface. We show that bacteriophage-encoded glucosylation of Shigella O antigen, the basis of different serotypes, shortens the LPS molecule by around half. This enhances TTSS function without compromising the protective properties of the LPS. Thus, LPS glucosylation promotes bacterial invasion and evasion of innate immunity, which may have contributed to the emergence of serotype diversity in Shigella .
- Published
- 2005
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