1. CD36 family members are TCR-independent ligands for CD1 antigen-presenting molecules
- Author
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Matthew E. Ritchie, Samuel J. Redmond, Adam P Uldrich, Catriona V. Nguyen-Robertson, Katherine H. A. Gourley, Catarina F. Almeida, D. Branch Moody, Hamish E G McWilliam, Shihan Li, Dale I. Godfrey, Nicholas A Gherardin, Fiona Ross, Daniel G. Pellicci, Robert De Rose, Rebecca Seneviratna, Jose A Villadangos, and Shian Su
- Subjects
CD36 Antigens ,T cell ,Immunology ,Primary Cell Culture ,CD1 ,Receptors, Antigen, T-Cell ,chemical and pharmacologic phenomena ,Ligands ,Article ,Antigens, CD1 ,03 medical and health sciences ,Jurkat Cells ,0302 clinical medicine ,Antigen ,Tetramer ,T-Lymphocyte Subsets ,parasitic diseases ,medicine ,Humans ,Receptor ,030304 developmental biology ,Glycoproteins ,0303 health sciences ,Antigen Presentation ,biology ,Chemistry ,T-cell receptor ,hemic and immune systems ,General Medicine ,Natural killer T cell ,Lipids ,Healthy Volunteers ,3. Good health ,Cell biology ,medicine.anatomical_structure ,CD1D ,Blood Buffy Coat ,biology.protein ,Protein Multimerization ,030215 immunology - Abstract
CD1c presents lipid-based antigens to CD1c-restricted T cells, which are thought to be a major component of the human T cell pool. However, the study of CD1c-restricted T cells is hampered by the presence of an abundantly expressed, non-T cell receptor (TCR) ligand for CD1c on blood cells, confounding analysis of TCR-mediated CD1c tetramer staining. Here, we identified the CD36 family (CD36, SR-B1, and LIMP-2) as ligands for CD1c, CD1b, and CD1d proteins and showed that CD36 is the receptor responsible for non-TCR-mediated CD1c tetramer staining of blood cells. Moreover, CD36 blockade clarified tetramer-based identification of CD1c-restricted T cells and improved identification of CD1b- and CD1d-restricted T cells. We used this technique to characterize CD1c-restricted T cells ex vivo and showed diverse phenotypic features, TCR repertoire, and antigen-specific subsets. Accordingly, this work will enable further studies into the biology of CD1 and human CD1-restricted T cells.
- Published
- 2021