1. Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo.
- Author
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Gurevich DB, Nguyen PD, Siegel AL, Ehrlich OV, Sonntag C, Phan JM, Berger S, Ratnayake D, Hersey L, Berger J, Verkade H, Hall TE, and Currie PD
- Subjects
- Animals, Animals, Genetically Modified, Cell Division genetics, Clone Cells, Muscle Development genetics, Muscle Development physiology, Muscle, Skeletal embryology, Muscle, Skeletal injuries, Mutation, Myogenic Regulatory Factor 5 genetics, Myogenin genetics, Regeneration genetics, Satellite Cells, Skeletal Muscle cytology, Transgenes, Zebrafish, Cell Division physiology, Cell Tracking methods, Muscle, Skeletal physiology, Regeneration physiology, Satellite Cells, Skeletal Muscle physiology
- Abstract
Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation-competent myoblasts. However, this model currently lacks in vivo validation. We define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for the asymmetric division of satellite cells driving both self-renewal and regeneration via a clonally restricted progenitor pool., (Copyright © 2016, American Association for the Advancement of Science.)
- Published
- 2016
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