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Only a few intracellular S-nitrosylated proteins have been identified, and it is unknown if protein S-nitrosylation/denitrosylation is a component of signal transduction cascades. Caspase-3 zymogens were found to be S-nitrosylated on their catalytic-site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway. Decreased caspase-3 S-nitrosylation was associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active-site thiol. Protein S-nitrosylation/denitrosylation can thus serve as a regulatory process in signal transduction pathways.

A depth-age scale and an accumulation history for the Holocene have been established on the Greenland Ice Sheet Project 2 (GISP2) deep core, providing the most continuously dated record of annual layer accumulation currently available. The depth-age scale was obtained with the use of various independent techniques to count annual layers in the core. An annual record of surface accumulation during the Holocene was obtained by correcting the observed layer thicknesses for flow-thinning. Fluctuations in accumulation provide a continuous and detailed record of climate variability over central Greenland during the Holocene. Climate events, including "Little Ice Age" type events, are examined.

Sulfate concentrations from continuous biyearly sampling of the GISP2 Greenland ice core provide a record of potential climate-forcing volcanism since 7000 B.C. Although 85 percent of the events recorded over the last 2000 years were matched to documented volcanic eruptions, only about 30 percent of the events from 1 to 7000 B.C. were matched to such events. Several historic eruptions may have been greater sulfur producers than previously thought. There are three times as many events from 5000 to 7000 B.C. as over the last two millennia with sulfate deposition equal to or up to five times that of the largest known historical eruptions. This increased volcanism in the early Holocene may have contributed to climatic cooling.

High-resolution, continuous multivariate chemical records from a central Greenland ice core provide a sensitive measure of climate change and chemical composition of the atmosphere over the last 41,000 years. These chemical series reveal a record of change in the relative size and intensity of the circulation system that transported air masses to Greenland [defined here as the polar circulation index (PCI)] and in the extent of ocean ice cover. Massive iceberg discharge events previously defined from the marine record are correlated with notable expansions of ocean ice cover and increases in PCI. During stadials without discharge events, ocean ice cover appears to reach some common maximum level. The massive aerosol loadings and dramatic variations in ocean ice cover documented in ice cores should be included in climate modeling.

It is estimated that 84 percent of the ice exiting the Arctic Basin through Fram Strait during June and July 1984 was multiyear ice and that a large percentage of this ice is ridged or otherwise deformed. While freeboard and thickness data, together with salinity measurements on cores, usually sufficed to distinguish between first and multiyear floes, preliminary identification could usually be made on the basis of snow cover measurements with snow cover being much thicker on multiyear ice. Cores from the top half meter of multiyear floes were generally very much harder and more transparent than cores from first-year floes. Age estimates of multiyear floes, based on petrographic and salinity characteristics of cores, did not exceed 4 to 5 years for any of the floes that were observed exiting Fram Strait.

Oxygen- and hydrogen-isotope analyses from the core hole through the Antarctic Ice Sheet at Byrd Station define temperature variations over more than 75,000 years. Synchronism between major climatic changes in Antarctica and the Northern Hemisphere is strongly indicated. The Wisconsin cold interval extended from 75,000 to 11,000 years ago. Three intra-Wisconsin warmer phases were all colder than pre- or post-Wisconsin times, which suggests that North American and Eurasian continental ice sheets did not disappear at any time during the Wisconsin.

The Antarctic ice sheet at Byrd Station has been core-drilled to bedrock; the vertical thickness of the ice is 2164 meters. Liquid water-indicative of pressure melting-was encountered at the bed. Heat flow through the base of the ice sheet is estimated at 1.8 microcalories per square centimeter per second. The minimum temperature was -28.8 degrees C at 800 meters; maximum ice density, 0.9206 at 1000 meters. Core studies reveal the existence of a chemically pure, structurally stratified sheet comprising bubbly ice to 900 meters that transforms to bubble-free deformed ice, with substantially vertically orientated c-axis structure, below 1200 meters. Below 1800 meters the deformed ice structure gives way to large annealed crystals. Several thin layers of dirt between 1300 and 1700 meters are tentatively identified as volcanic ash, and horizontally banded debris, including fragments of granite, is present in the basal ice.