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Based on the new syntrophic methanogenesis route via direct interspecies electron transfer (DIET), a novel bioaugmentation method by adding exoelectrogenic Geobacter species to accelerate methanogenesis was developed in this study. Geobacter sulfurreducens PCA, type exoelectrogenic strain of Geobacter species was chosen for the research. To clarify the effect of G. sulfurreducens on methanogenesis, batch tests of CH 4 production were carried out. Acetate, the most typical precursor of methanogenesis was chosen as the substrate of batch tests. Amendment of G. sulfurreducens accelerated CH 4 production remarkably. The lag phase of CH 4 production was shortened, and the maximum CH 4 production rate was increased by 78%. Fluorescence in situ hybridization showed that G. sulfurreducens closely gathered with methanogens. For the archaeal communities, the high-throughput sequencing results demonstrated that Methanosaetaceae and Methanobacteriaceae were potential bioaugmented methanogens. We speculated that the accelerated methanogenesis by adding G. sulfurreducens may result from the syntrophic association between G. sulfurreducens and methanogens affiliated with Methanosaetaceae and Methanobacteriaceae . This research provides a new route to enhance methanogenesis through the utilization of G. sulfurreducens . Through this study, the role of Geobacter in the anaerobic engineering and carbon cycling of nature should be paid more attention. [ABSTRACT FROM AUTHOR]

Anaerobic digestion is an effective biological treatment process that produces methane by degrading organic compounds in waste/wastewater. It is a complicated microbial process by metabolic interactions among different types of microorganisms. In this process, efficient interspecies electron transfer between secondary fermenting bacteria and methanogens is the critical process for fast and effective methanogenesis. In syntrophic metabolism, hydrogen or formate has been considered as the conventional electron carrier transferring electrons from secondary fermenting bacteria to hydrogenotrophic methanogens. Recently, direct interspecies electron transfer (DIET) without the involvement of dissolved redox mediators is arousing great concerns and has been regarded as a more efficient and thermodynamically favorable interspecies electron transfer pathway for methanogenesis. Interspecies electron exchange through DIET is accomplished via the membrane-bound cytochromes or conductive pili. Several kinds of exogenously-added conductive or semiconductive iron oxides have been discovered to greatly enhance anaerobic methanogenesis through promoting DIET. Different (semi)conductive iron oxides give a boost to DIET through different mechanisms based on the physicochemical properties of the iron oxides and the reciprocal interactions between iron oxides and functional microorganisms. In this review, the current understanding of interspecies electron transfer in syntrophic-methanogenic consortions is summarized, the effects and deep-rooted mechanisms of (semi)conductive iron oxides on methanogenesis and DIET are discussed, and possible future perspectives and development directions are suggested for DIET via (semi)conductive iron oxides in anaerobic digestion. Unlabelled Image • Interspecies electron transfer is the critical step for syntrophic methanogenesis. • H 2 acts as diffusive electron carriers between syntrophic partners in IHT pathway. • DIET is established by biological electrical connections instead of diffusive H 2. • (Semi)conductive iron oxides promote syntrophic methanogenesis by enhancing DIET. • Electric microbial syntrophy network is built by (semi)conductive iron oxides. [ABSTRACT FROM AUTHOR]