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Algae CO2 biofixation provides a promising opportunity due to earn carbon credits and valuable end uses. For balancing technology, energy and economy issues in practical utilization, this approach quantitively interprets the contradictions from upstream CO2 source with a wide range of initial concentration to downstream CO2 biofixation product including edible algae and algal biomass. The influence of upstream CO2 deliverable on algal quantity and quality have been assessed, and the influence of CO2 concentration on CO2 transport mode choice has been also assessed coupling the transportation distance. In downstream algal fixation, quantitively relationship of algal growth have been established. The assessment discovered that direct energy consumptions complied with logarithmic relationship with specific productivities while both direct energy and indirect energy consumption complied with linear relationship with protein content. According to sensitive uncertainty analysis, initial CO2 concentration is a critical parameter to influence significantly energy consumption in upstream CO2 deliverables and algal quality while the contents of protein and specific productivity are the critical sensitive parameters in downstream algae deliverables. Potential modification systems are achieved for significantly reducing energy consumption by improving specific productivity and carbon abundance with low protein content in algae.