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Excess phosphorus (P) from agriculture is a leading cause of harmful and nuisance algal blooms in many freshwater ecosystems. Throughout much of the midwestern United States, extensive networks of subsurface tile drains remove excess water from fields and allow for productive agriculture. This enhanced drainage also facilitates the transport of P, particularly soluble reactive phosphorus (SRP), to adjacent streams and ditches, with harmful consequences. Thus, reducing SRP loss from tile-drained cropland is a major focus of regional and national efforts to curb eutrophication and algal blooms. The planting of cover crops after crop harvest is a conservation practice that has the potential to increase retention of fertilizer nutrients in watersheds by extending the growing season and limiting bare ground in the fallow season; however, the effect of cover crops on SRP loss is inconsistent at the field-scale and unknown at the watershed-scale. In this study, we conducted a large-scale manipulation of land cover in a small, agricultural watershed by planting cover crops on >60% of croppable acres for six years and examining changes in SRP loss through tile drains and at the watershed outlet. We found reduced median SRP loss from tiles with cover crops compared to those without cover crops, particularly during periods of critical export from January to June. Variation in tile discharge influenced SRP loss, but relationships were generally weaker in tiles with cover crops (i.e., decoupled) compared to tiles without cover crops. At the watershed outlet, SRP yield was highly variable over all seasons and years, which complicated efforts to detect a significant effect of changing land cover on SRP export to downstream systems. Yet, watershed-scale planting of cover crops slowed cumulative SRP losses and reduced SRP export during extreme events. Overall, this study demonstrates the potential for cover crops to alter patterns of SRP loss at both the field- and watershed-scale., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021. Published by Elsevier B.V.)

Floodplain restoration constructed via the two-stage ditch in agricultural streams has the potential to enhance nutrient retention and prevent the eutrophication of downstream ecosystems. Identifying the role of biotic and abiotic factors influencing soluble reactive phosphorus (SRP) retention in floodplains is of interest given that changing redox conditions associated with floodplain inundation can result in a release of geochemically sorbed SRP to the water column. In three agricultural waterways (Indiana, USA), we conducted seasonal measurements of a suite of biogeochemical pools (total P, bioavailable P and Fe) and processes (SRP flux and microbial respiration) from multiple floodplain transects, along with their adjacent stream sediments, to determine the role of biotic and abiotic processes on floodplain SRP retention or release. Across floodplain soils, organic matter explained a significant amount of variation in soil respiration, and SRP flux from the water column to the floodplain soils was driven by the molar ratio of Fe: P, with values >6 indicating potential SRP sorption due to increased available sorption sites. We developed a mass balance model at a single site to relate seasonal floodplain processes with water column SRP export, above and below the study reach, using measurements in this study combined with data from the literature. Grab sample data suggest that the reach retained 26% of incoming SRP, which the mass balance model attributed to seasonal synergy between plant assimilation in spring and summer (removing P from floodplain soils) and abiotic P sorption during winter and spring inundation (adding SRP to the floodplain). Retention of SRP was higher in floodplain soils compared to stream sediments based on the modeled SRP budget. Thus, we suggest that these constructed floodplains will maximize SRP retention from the water column if they inundate regularly, have floodplain soils with Fe:P > 3-6, and that promote sustained plant life., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The insecticidal Cry1Ab protein expressed by transgenic (Bt) maize can enter adjacent water bodies via multiple pathways, but its fate in stream ecosystems is not as well studied as in terrestrial systems. In this study, we used a combination of field sampling and laboratory experiments to examine the occurrence, leaching, and degradation of soluble Cry1Ab protein derived from Bt maize in agricultural streams. We surveyed 11 agricultural streams in northwestern Indiana, USA, on 6 dates that encompassed the growing season, crop harvest, and snowmelt/spring flooding, and detected Cry1Ab protein in the water column and in flowing subsurface tile drains at concentrations of 3-60ng/L. In a series of laboratory experiments, submerged Bt maize leaves leached Cry1Ab into stream water with 1% of the protein remaining in leaves after 70d. Laboratory experiments suggested that dissolved Cry1Ab protein degraded rapidly in microcosms containing water-column microorganisms, and light did not enhance breakdown by stimulating assimilatory uptake of the protein by autotrophs. The common detection of Cry1Ab protein in streams sampled across an agricultural landscape, combined with laboratory studies showing rapid leaching and degradation, suggests that Cry1Ab may be pseudo-persistent at the watershed scale due to the multiple input pathways from the surrounding terrestrial environment., (Copyright © 2017 Elsevier B.V. All rights reserved.)