The huge excrement quantity from the increasing large-scale livestock stressed the ecological, environmental deterioration. As a major benefit for handling livestock manure, the slurry of biogas (BS) is developed during the production of biogas that might increase plant productivity. However, nitrous oxide (N 2 O) emissions from BS are considered a significant danger to the environment due to global warming potential. Furthermore, applying different proportions of BS combined with chemical fertilizer (CF) on N 2 O productions in the North China Plain (NCP) remains unclear. Herein, two sequential field trials were performed by maize-wheat rotations to substitute the CF by BS and reduce N 2 O emissions while keeping the crop yield stable. Four treatments were conducted, including T1, T3, T6, and CK. A total of 226.5 kg N ha -1 used in the maize-wheat rotation system. Additionally, different ratios of BS (100%, 80%, and 50%) combined with CF were used in wheat season in the tillering stage. Results showed integrated applications of BS with CF have potential for reducing N 2 O emission. Our findings showed that the maximum grain yield of CF was 6250 kg ha -1 , which might be achieved by applying 38% BS and 62% of CF. This ratio yielded 1.03 kg ha -1 N 2 O emissions, which was 15% lesser than the N 2 O emission of CK, 1.21 kg ha -1 . Considering whole growing period of wheat biogas treatments significantly reduced the cumulative N 2 O emissions from 17% to 26% compared to CF. To achieve maximum yield and minimum N 2 O emissions, an optimum 38% BS ratio has been suggested. The integrated use of BS and CF provided the greatest grain yield because of necessary nutrients provided by both slurry and CF. Consequently, N 2 O emissions reduced based on frequency and type of fertilizer. In conclusion, 38% ratio of BS combined with 62% CF would be a suitable approach to mitigate N 2 O emission and simultaneously increase crop yield in NCP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)