3 results on '"biological methods"'
Search Results
2. Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions
- Author
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Beñat Zaldibar, Jon Sáenz, Xabier Lekube, Manu Soto, Denis Benito, Urtzi Izagirre, Aino Ahvo, Jari Nuutinen, Ionan Marigómez, Dennis Bilbao, Kari K. Lehtonen, Nestor Etxebarria, and European Commission more...
- Subjects
Baltic States ,digestive cells ,010504 meteorology & atmospheric sciences ,Pilot Projects ,biomarkkerit ,010501 environmental sciences ,surface temperature ,01 natural sciences ,biological methods ,baltic sea ,Environmental monitoring ,Biomonitoring ,blue mussels ,immune-responses ,Kiel ,experimental exposure ,Waste Management and Disposal ,biology ,Ecology ,Mytilus trossulus ,reference values ,tracking ,Pollution ,Mytilus ,edulis l ,macoma-balthica ,Macoma balthica ,Environmental Monitoring ,Environmental Engineering ,Baltic Sea ,Tvärminne ,lipid-peroxidation ,medicine ,Environmental Chemistry ,Animals ,14. Life underwater ,seuranta ,biologiset menetelmät ,0105 earth and related environmental sciences ,particulate organic-carbon ,bivalvia ,biomarkers ,Mussel ,galloprovincialis ,Seasonality ,biology.organism_classification ,medicine.disease ,simpukat ,marine research ,Salinity ,13. Climate action ,merentutkimus ,biomonitoring ,Environmental science ,Water Pollutants, Chemical - Abstract
For reliable mussel monitoring programmes based on biomarkers, regionally relevant reference values and their natural variability need to be known. The Baltic Sea exhibits high inter-regional and seasonal variability in physical factors such as salinity, temperature and primary production. The aim of this pilot study is to depict the effects of season-related environmental factors in a selected battery of biomarkers in two environmentally different sub-regions of the Baltic Sea to help establishing reference data for biochemical, cellular and tissue-level biomarkers. In order to achieve that, mussels were collected from reference sites in Kiel (Germany) and Tvarminne (Finland) during three seasons: summer and autumn 2016, and spring 2017. Finally, in order to characterize the ecological situation, analysis of the chemical tissue burden was performed and chlorophyll a and particulate organic carbon concentration and temperature changes were analyzed at each sampling locality using satellite remote sensing images. An integrated biomarker response index was performed to summarize the biomarker responses of each locality and season. The biochemical endpoints showed seasonal variability regulated by temperature, food supply and reproductive cycle, while among the cellular endpoints only lipofuscin accumulation and lysosomal structural changes showed slight seasonal variation. Seasonal changes in tissue level biomarkers were observed only at the northern Baltic Sea site Tvarminne, dictated by the demanding energetic trade-off caused by reproduction. In conclusion, the characterization of the ecological variables and physico-chemical conditions at each site, is crucial to perform a reliable assessment of the effects of a hypothetical pollution scenario in the Baltic Sea. Moreover, reference levels of biomarkers and their responses to natural environmental conditions must be established. (C) 2019 The Authors. Published by Elsevier B.V. This work was funded by EU GRACE Project (Grant Agreement Number 679266), grant CGL2016-76561-R (MINECO/ERDF, UE). Additional funding was received from the University of the Basque Country (UPV/EHU, UFI 11/37 fellowship, GIU 17/002) and Basque Government (IT810-13). Satellite data were provided by NASA Goddard Space Flight Center, Ocean Ecology Laboratory, Ocean Biology Processing Group; 2018, Ocean Color Data, NASA OB. DAAC. Accessed on 2018/10/29. Dr. Matthias Brenner (Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany) is gratefully acknowledged for the sampling and preparation of mussels collected from the German coast. more...
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- 2019
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3. Effects of contaminated surface water and groundwater from a rare earth mining area on the biology and the physiology of Sprague-Dawley rats.
- Author
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He, Xiaoying, Yuan, Ting, Jiang, Xinying, Yang, Hui, and Zheng, Chun Li
- Abstract
Previous studies have shown that an effective damage detection method for model rats from macro individual to micro cellular, was applied to assess the groundwater quality from rare earth metals tailings seepage. To determine whether it is universal method for measuring the toxicological damage caused by contaminated water around other mining areas to organisms at the organ-tissue-cell-chromosome-gene level. In this study, a rare earth mining area in North China was used as research base. Firstly, the core pollution factors in surface water and groundwater from five different sites were analyzed. Then, the degree of toxicological damage to Sprague-Dawley (SD) rats caused by contaminated water were systematically assessed using biological methods. Finally, the possible molecular mechanism of toxicological damage was further discussed. The synthesis results showed that the main pollution factors were some metal elements (Mn, Zn, Co, Ni) and rare earth elements (Sc, Nb, La, Ce, Pr, Dy and Y), which might cause significant DNA genetic damage to SD rats. Further, differential gene expression profile showed that DNA damage-inducible genes (Gadd45g and Ddit4), immunity-related genes (Mpo, Slpi and Elane) and two cancer-related genes (Mmp8 and Ltf) were used as a new prognostic and predictive biomarker for biosafety assessment. Therefore, this study provides a possible molecular mechanism for the toxicological damage, and also it provides a universal method to scientifically and effectively evaluate the water pollution risk for other mining areas. Unlabelled Image • A universal method for assessment water quality were established. • Mn, Zn, Co, Ni, Sc, Nb, La, Ce, Pr, Dy and Y might cause DNA damage to SD rats. • Gadd45g and Ddit4 might be used as new biomarkers for biosafety assessment. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
- Full Text
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