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Start Over Author "Ma, Fengwang" Journal scientia horticulturae
45 results on '"Ma, Fengwang"'

23. Validation of reference genes for qRT-PCR analysis in peel and flesh of six apple cultivars (Malus domestica) at diverse stages of fruit development

Author

Lifang Zhu, Ma FengWang, Nannan Wang, Cuiying Li, Yaohua You, Chengquan Yang, and Wei Liang

Subjects
0106 biological sciences, 0301 basic medicine, Malus, Candidate gene, biology, Flesh, food and beverages, Horticulture, biology.organism_classification, 01 natural sciences, law.invention, 03 medical and health sciences, 030104 developmental biology, law, Reference genes, Gene expression, Cultivar, Gene, Polymerase chain reaction, 010606 plant biology & botany
Abstract

Apple (Malus domestica), as one of world’s significant fruits, color of fruit contributes a lot for its high economic benefits and edible values. Quantitative real-time polymerase chain reaction (qRT-PCR) is a technique in molecular biology commonly used for the analysis of gene expression. Actually, stability of reference genes used in data normalization affects the accuracy and reliability of qRT-PCR to a large extend. Therefore, the present study was designed to identify and validate the suitable reference genes during the fruit development. We evaluated the stability of 10 candidate reference genes in peel or flesh samples of six cultivars during different development conditions. The qRT-PCR results showed that amplification efficiency of all candidate genes ranged from 95 to 111.3%. Three software packages geNorm, NormFinder, and BestKeeper recommended different reference genes in one same sample sets. So, an online tool RefFinder was applied for integrating above three results and then these 10 reference genes were ranked comprehensively. According to RefFinder, for all fruit development conditions, WD40, ACT and GAPDH were identified as the most reliable reference genes for apple peel; EF-1α, CKL and WD40 for apple flesh, and MDH was the least stable reference gene for both samples. And, the expression patterns of target gene MdUFGT also validated this result. Further, we validated the suggested combination of different genes. Through the selection and validation of these internal reference genes, the accuracy and reliability of gene expression analysis in apple fruit tissues can be improved.

Published
2019

30. Plant regeneration from in vitro cultured leaves of Lanzhou lily (Lilium davidii var. unicolor)

Author

Xu LingFei, Liang Dong, and Ma FengWang

Subjects
biology, Micropropagation, Lilium, Liliaceae, Callus, Lilium davidii, Botany, Shoot, Horticulture, biology.organism_classification, Explant culture, Somaclonal variation
Abstract

Lanzhou lily ( Lilium davidii var. unicolor) is one of the best lilies which are edible in China but the efficient shoot regeneration system has not been developed. The purpose of the present study is to establish an efficient and reproducible protocol for induction of shoots in vitro from L. davidii var. unicolor leaves. Shoot regeneration from in vitro cultured leaves of L. davidii var. unicolor was tested on the 26 media based on NN [Nitsch, J.P., Nitsch, C., 1969. Haploid plants from pollen grains. Science 163, 85–87] basal medium, containing different concentrations of thidiazuron (TDZ) in combination with different concentrations of α-naphthaleneacetic acid (NAA). Shoot organogenesis occurred directly from the leaves without forming callus. Shoot regeneration mainly occurred from the cuts across the midvein and the base of the leaf explants. The highest frequency of regeneration (93.3%) and the largest number of shoots per leaf (3.83) were obtained on NN basal medium supplemented with 0.5 mg l −1 TDZ and 1.0 mg l −1 NAA. All the regenerated shoots formed complete plantlets on half-strength MS [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15, 473–497] basal medium containing 0.1–0.5 mg l −1 indole-3-butyric acid (IBA) with in 30 days, and 92% of the regenerated plantlets survived in the soil. This study will be useful for Agrobacterium -mediated transformation and exploitation of somaclonal variation of Lanzhou lily.

Published
2009

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