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Nowadays, medicinal herbs with an adaptogenic effect to human body start be very popular not only in medicine but also in food industry due to the high amount of bioactive compounds. The aim of this study was to determine antioxidant activity, total polyphenol, flavonoid and phenolic acid content of five plants with adaptogenic affect to human body: Eurycoma longifolia Jack., Lepidium meyenii Walp., Turnera diffusa Wild., Tribulus terrestris L. and Rhodiola rosea L. The antioxidant activity was detected by DPPH and phosphomolybdenum method, total polyphenol content with Folin – Ciocalteu reagent, flavonoids content by aluminium chloride method and total phenolic acid content by Arnova reagent. The highest antioxidant activity by DPPH and phosphomolybdenum method was determined in the sample of Rhodiola rosea L. (6.82 mg TEAC – Trolox equivalent antioxidant capacity per g of sample and 315.37 mg TEAC per g). In this sample was also measured the highest content of total polyphenols (76.64 mg GAE – gallic acid equivalent per g of sample) and phenolic acid (25.10 mg CAE – caffeic acid equivalent per g of sample). Total flavonoid content (20.80 mg QE – quercetin equivalent per g of sample) was the highest in sample of Tribulus terrestris L. Results showed that medicinal herbs with adaptogenic effect are rich for polyphenol compounds with antioxidant activity and can be use more in food industry as functional food additives.