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This study focuses on the significance of improving the land degradation of Mu Us Sandy Land to increase cultivated land area and promote ecological green development. The research objects were four kinds of mixed soils, and rhizosphere soils were collected during the crop harvesting period. The volume ratio of soft rock to sand was 0:1 (control check, CK), 1:5 (composite soil one, PS1), 1:2 (composite soil two, PS2), and 1:1 (composite soil three, PS3). The results showed that the large aggregates were primarily mechanically stable aggregates, while the small aggregates were mainly water-stable aggregates. The soft rock promoted the increase of clay and silt content in sandy soil, and the soil texture changed from sand to loam. The contents of organic matter, available phosphorus, and available potassium increased significantly under PS2 and PS3 treatments, but there was no significant difference between them. Total nitrogen had no significant difference among treatments. Actinobaciota, Proteobateria, and Chloroflexi were the dominant bacteria in rhizosphere soil, accounting for about 75% of all microorganisms. At the Genus level, the soft rock contributes to richer species composition. The diversity index, evenness index, and richness index was higher in PS1, and the available phosphorus and available potassium content promoted the increase of diversity. Therefore, when the proportion of soft rock and sand compound soil is between 1: 5 and 1: 2, it can be used as an important basis and technical parameter for Mu Us Sandy Land improvement. [ABSTRACT FROM AUTHOR]

Plant growth environment plays an important role in shaping soil microbial communities. To understand the response of soil rhizosphere microbial communities in Oplopanax elatus Nakai plant to a changed growth conditions from natural habitation to cultivation after transplant. Here, a comparative study of soil chemical properties and microbial community using high-throughput sequencing was conducted under cultivated conditions (CT) and natural conditions (WT), in Changbai Mountain, Northeast of China. The results showed that rhizosphere soil in CT had higher pH and lower content of soil organic matter (SOM) and available nitrogen compared to WT. These changes influenced rhizosphere soil microbial communities, resulting in higher soil bacterial and fungi richness and diversity in CT soil, and increased the relative abundance of bacterial phyla Acidobacteria, Chloroflexi, Gemmatimonadetes, Firmicutes and Patescibacteria, and the fungi phyla Mortierellomycota and Zoopagomycota, while decreased bacterial phyla Actinobacteria, WPS-2, Gemmatimonadetes, and Verrucomicrobia, and the fungi phyla Ascomycota, and Basidiomycota. Redundancy analysis analysis indicated soil pH and SOM were the primarily environmental drivers in shaping the rhizosphere soil microbial community in O. elatus under varied growth conditions. Therefore, more attention on soil nutrition management especially organic fertilizer inputs should be paid in O. elatus cultivation. [ABSTRACT FROM AUTHOR]