1. A combined method to quantify the retinal metabolic rate of oxygen using photoacoustic ophthalmoscopy and optical coherence tomography
- Author
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Nader Sheibani, Amani A. Fawzi, Wei Song, Wenzhong Liu, Qing Wei, Robert A. Linsenmeier, Ji Yi, Shuliang Jiao, Hao Zhang, and Tan Liu
- Subjects
Pathology ,medicine.medical_specialty ,Glaucoma ,01 natural sciences ,Article ,Retina ,010309 optics ,Ophthalmoscopy ,Photoacoustic Techniques ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Hemoglobins ,0302 clinical medicine ,Optical coherence tomography ,0103 physical sciences ,medicine ,Animals ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,Retinal ,Diabetic retinopathy ,Blood flow ,medicine.disease ,Rats ,Oxygen ,medicine.anatomical_structure ,chemistry ,030221 ophthalmology & optometry ,Tomography ,Basal Metabolism ,business ,Blood Flow Velocity ,Tomography, Optical Coherence ,Biomedical engineering - Abstract
Quantitatively determining physiological parameters at a microscopic level in the retina furthers the understanding of the molecular pathways of blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is the retinal oxygen metabolic rate (rMRO2). Quantifying rMRO2 is challenging because two parameters, the blood flow rate and hemoglobin oxygen saturation (sO2), must be measured together. We combined photoacoustic ophthalmoscopy (PAOM) with spectral domain-optical coherence tomography (SD-OCT) to tackle this challenge, in which PAOM measured the sO2 and SD-OCT mapped the blood flow rate. We tested the integrated system on normal wild-type rats, in which the measured rMRO2 was 297.86 ± 70.23 nl/minute. This quantitative method may shed new light on both fundamental research and clinical care in ophthalmology in the future.
- Published
- 2014