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Your search keyword '"Buchfelder, Michael"' showing total 14 results
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14 results on '"Buchfelder, Michael"'

3. Expression of SRY-related HMG Box Transcription Factors (Sox) 2 and 9 in Craniopharyngioma Subtypes and Surrounding Brain Tissue

Author

Thimsen, Vivian, John, Nora, Buchfelder, Michael, Flitsch, Jörg, Fahlbusch, Rudolf, Stefanits, Harald, Knosp, Engelbert, Losa, Marco, Buslei, Rolf, Hölsken, Annett, Thimsen, Vivian, John, Nora, Buchfelder, Michael, Flitsch, Jörg, Fahlbusch, Rudolf, Stefanits, Harald, Knosp, Engelbert, Losa, Marco, Buslei, Rolf, and Hölsken, Annett

Subjects
Adult, Male, animal structures, Adolescent, lcsh:Medicine, Article, Craniopharyngioma, Young Adult, stomatognathic system, Medizinische Fakultät, Biomarkers, Tumor, Tumor Microenvironment, Humans, Pituitary Neoplasms, ddc:610, RNA, Messenger, lcsh:Science, Child, beta Catenin, Aged, Multidisciplinary, SOXB1 Transcription Factors, lcsh:R, Brain, Infant, SOX9 Transcription Factor, Middle Aged, Immunohistochemistry, Gene Expression Regulation, Neoplastic, Child, Preschool, Neoplastic Stem Cells, lcsh:Q, Female
Abstract

Stem cells have been discovered as key players in the genesis of different neoplasms including craniopharyngioma (CP), a rare tumour entity in the sellar region. Sox2 and Sox9 are well-known stem cell markers involved in pituitary development. In this study we analysed the expression of both transcription factors using immunohistochemistry in a large cohort of 64 adamantinomatous (aCP) and 9 papillary CP (pCP) and quantitative PCR in 26 aCP and 7 pCP. Whereas immunohistochemically Sox2+ cells were verifiable in only five aCP (7.8%) and in 39.1% of the respective surrounding cerebral tissue, pCP specimens appeared always negative. In contrast, Sox9 was detectable in all tumours with a significantly higher expression in aCP compared to pCP (protein, p

Published
2017

4. EpCAM (CD326) is differentially expressed in craniopharyngioma subtypes and Rathke’s cleft cysts

Author

Thimsen, Vivian, Hölsken, Annett, Buchfelder, Michael, Flitsch, Jörg, Fahlbusch, Rudolf, Stefanits, Harald, Losa, Marco, Jones, David T. W., and Buslei, Rolf

Subjects
Adult, Male, Adolescent, Gene Expression Profiling, Middle Aged, Epithelial Cell Adhesion Molecule, Article, Craniopharyngioma, Young Adult, Medizinische Fakultät, Child, Preschool, Biomarkers, Tumor, Humans, Female, Pituitary Neoplasms, ddc:610, Central Nervous System Cysts, Child, Aged
Abstract

The epithelial cell adhesion molecule (EpCAM) is a type I glycoprotein located on the surface of epithelial cells. It is strongly expressed in many neoplasms and already used in the diagnosis and distinction of various tumour subtypes. Comparative studies about EpCAM expression in cystic sellar lesions are lacking. Therefore, we analysed its distribution pattern in adamantinomatous (aCP) and papillary (pCP) craniopharyngiomas (CP) and Rathke's Cleft Cysts (RCC) using immunohistochemistry and gene expression profiling. Whereas the protein was not detectable in pCP (n = 10), all aCP (n = 64) showed distinct staining patterns. The vast majority of RCC (n = 10) also appeared positive, but these displayed notably lower labeling scores. Additionally, significantly higher mRNA levels were detectable in aCP (n = 19) when compared to pCP (n = 10) (p = 9.985(-8)). Furthermore, pediatric aCP cases, in general, exhibited stronger EpCAM staining levels compared to adult ones (p = 0.015). However, we were not able to verify this result on mRNA level. In summary, our findings demonstrate that EpCAM can be used as an additional distinction-marker for cystic lesions of the sellar region. Its unknown function in aCP and the presence of an approved monoclonal bispecific trifunctional antibody for cancer therapy are interesting starting points for further studies.

Published
2016
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6. Correction: Corrigendum: Identification of two novel Chlorotoxin derivatives CA4 and CTX-23 with chemotherapeutic and anti-angiogenic potential

Author

Xu, Tengfei, primary, Fan, Zheng, additional, Li, Wenxin, additional, Dietel, Barbara, additional, Wu, Yingliang, additional, Beckmann, Matthias W., additional, Wrosch, Jana K., additional, Buchfelder, Michael, additional, Eyupoglu, Ilker Y., additional, Cao, Zhijian, additional, and Savaskan, Nicolai E., additional

Published
2016
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