6 results on '"Han Tang"'
Search Results
2. Bronchial epithelium repair by Esculentin-1a-derived antimicrobial peptides: involvement of metalloproteinase-9 and interleukin-8, and evaluation of peptides’ immunogenicity
- Author
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Danilo Ranieri, Floriana Cappiello, Loretta Ferrera, Maria Luisa Mangoni, Han-Tang Chen, Y. Peter Di, Bruno Casciaro, and Veronica Carnicelli
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Lipopolysaccharides ,Male ,Cell biology ,cell migration ,antimicrobial peptide ,Antimicrobial peptides ,lcsh:Medicine ,Bronchi ,Matrix metalloproteinase ,Matrix Metalloproteinase Inhibitors ,Article ,Antibodies ,Epithelium ,Cell Line ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Movement ,Animals ,Humans ,metalloproteinase ,wound healing ,Interleukin 8 ,Glycosides ,RNA, Messenger ,lcsh:Science ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Metalloproteinase ,Wound Healing ,Multidisciplinary ,Chemistry ,Immunogenicity ,lcsh:R ,Interleukin-8 ,Cell migration ,Chemical biology ,3. Good health ,Matrix Metalloproteinase 9 ,030220 oncology & carcinogenesis ,Pregnenolone ,Respiratory epithelium ,lcsh:Q ,Female ,Wound healing ,Peptides ,Antimicrobial Cationic Peptides - Abstract
The airway epithelium is seriously damaged upon pulmonary Pseudomonas aeruginosa infection, especially in cystic fibrosis (CF) sufferers. Therefore, the discovery of novel anti-infective agents accelerating healing of infected injured tissues is crucial. The antipseudomonal peptides esculentin-1a(1–21)NH2 and its diastereomer Esc(1–21)-1c (Esc peptides) hold promise in this respect. In fact, they stimulate airway epithelial wound repair, but no mechanistic insights are available. Here we demonstrated that this process occurs through promotion of cell migration by an indirect activation of epidermal growth factor receptor mediated by metalloproteinases. Furthermore, we showed an increased expression of metalloproteinase 9, at both gene and protein levels, in peptide-treated bronchial epithelial cells with a functional or mutated form of CF transmembrane conductance regulator. In addition, the two peptides counteracted the inhibitory effect of Pseudomonas lipopolysaccharide (mimicking an infection condition) on the wound healing activity of the airway epithelium, and they enhanced the production of interleukin-8 from both types of cells. Finally, no immunogenicity was discovered for Esc peptides, suggesting their potential safety for clinical usage. Besides representing a step forward in understanding the molecular mechanism underlying the peptide-induced wound healing activity, these studies have contributed to highlight Esc peptides as valuable therapeutics with multiple functions.
- Published
- 2019
3. Computer aided protein engineering to enhance the thermo-stability of CXCR1- T4 lysozyme complex
- Author
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Zeyu Jin, Lei-Han Tang, Lanqing Huang, Weontae Lee, Ji Hye Yun, Jaehyun Park, Yang Wang, Haiguang Liu, Xuanxuan Li, and Cecylia S. Lupala
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0301 basic medicine ,In silico ,Protein design ,Mutant ,lcsh:Medicine ,Computational biology ,Plasma protein binding ,Molecular Dynamics Simulation ,Protein Engineering ,Article ,Receptors, Interleukin-8A ,03 medical and health sciences ,Structure-Activity Relationship ,0302 clinical medicine ,Protein Interaction Domains and Motifs ,Homology modeling ,Amino Acid Sequence ,lcsh:Science ,Thermostability ,G protein-coupled receptor ,Multidisciplinary ,Binding Sites ,Chemistry ,Protein Stability ,lcsh:R ,Protein engineering ,Molecular Docking Simulation ,030104 developmental biology ,Mutation ,Thermodynamics ,lcsh:Q ,Muramidase ,030217 neurology & neurosurgery ,Protein Binding - Abstract
CXCR1, a member in G-protein coupled receptor (GPCR) family, binds to chemokine interleukin-8 (IL-8) specifically and transduces signals to mediate immune and inflammatory responses. Despite the importance of CXCR1, high-resolution structure determination is hindered by the challenges in crystallization. It has been shown that properly designed mutants with enhanced thermostability, together with fusion partner proteins, can be useful to form crystals for GPCR proteins. In this study, in silico protein design was carried out by using homology modeling and molecular dynamics simulations. To validate the computational modeling results, the thermostability of several mutants and the wild type were measured experimentally. Both computational results and experimental data suggest that the mutant L126W has a significant improvement in the thermostability. This study demonstrated that in silico design can guide protein engineering and potentially facilitate protein crystallography research.
- Published
- 2018
4. In-Situ Probing Plasmonic Energy Transfer in Cu(In, Ga)Se2 Solar Cells by Ultrabroadband Femtosecond Pump-Probe Spectroscopy
- Author
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Atsushi Yabushita, Jenh-Yih Juang, Yu-Lun Chueh, Shih Han Tang, Chih-Wei Luo, Kaung-Hsiung Wu, Shih Chen Chen, Hao-Chung Kuo, and Jia Xing Li
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Multidisciplinary ,Materials science ,business.industry ,Copper indium gallium selenide solar cells ,Article ,Electron transfer ,Excited state ,Femtosecond ,Optoelectronics ,Plasmonic solar cell ,Surface plasmon resonance ,Absorption (electromagnetic radiation) ,business ,Plasmon - Abstract
In this work, we demonstrated a viable experimental scheme for in-situ probing the effects of Au nanoparticles (NPs) incorporation on plasmonic energy transfer in Cu(In, Ga)Se2 (CIGS) solar cells by elaborately analyzing the lifetimes and zero moment for hot carrier relaxation with ultrabroadband femtosecond pump-probe spectroscopy. The signals of enhanced photobleach (PB) and waned photoinduced absorption (PIA) attributable to surface plasmon resonance (SPR) of Au NPs were in-situ probed in transient differential absorption spectra. The results suggested that substantial carriers can be excited from ground state to lower excitation energy levels, which can reach thermalization much faster with the existence of SPR. Thus, direct electron transfer (DET) could be implemented to enhance the photocurrent of CIGS solar cells. Furthermore, based on the extracted hot carrier lifetimes, it was confirmed that the improved electrical transport might have been resulted primarily from the reduction in the surface recombination of photoinduced carriers through enhanced local electromagnetic field (LEMF). Finally, theoretical calculation for resonant energy transfer (RET)-induced enhancement in the probability of exciting electron-hole pairs was conducted and the results agreed well with the enhanced PB peak of transient differential absorption in plasmonic CIGS film. These results indicate that plasmonic energy transfer is a viable approach to boost high-efficiency CIGS solar cells.
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- 2015
5. Obesity Leads to Tissue, but not Serum Vitamin A Deficiency
- Author
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Jose Jessurun, Steven E. Trasino, Lorraine J. Gudas, and Xiao-Han Tang
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Vitamin ,Male ,medicine.medical_specialty ,Mice, Obese ,030209 endocrinology & metabolism ,Biology ,Diet, High-Fat ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Weight loss ,Internal medicine ,medicine ,Animals ,Obesity ,Vitamin A ,030304 developmental biology ,Adiposity ,2. Zero hunger ,0303 health sciences ,Multidisciplinary ,Vitamin A Deficiency ,Fatty liver ,Retinol ,medicine.disease ,Vitamin A deficiency ,Fatty Liver ,Mice, Inbred C57BL ,Retinoic acid receptor ,Endocrinology ,chemistry ,Liver ,Hepatic stellate cell ,Steatosis ,medicine.symptom - Abstract
Obesity negatively affects multiple metabolic pathways, but little is known about the impact of obesity on vitamin A (VA)[retinol (ROL)], a nutrient that regulates expression of genes in numerous pathways essential for human development and health. We demonstrate that obese mice, generated from a high fat diet (HFD) or by genetic mutations (i.e., ob/ob; db/db), have greatly reduced ROL levels in multiple organs, including liver, lungs, pancreas and kidneys, even though their diets have adequate VA. However, obese mice exhibit elevated serum VA. Organs from obese mice show impaired VA transcriptional signaling, including reductions in retinoic acid receptor (RARα, RARβ2 and RARγ) mRNAs and lower intracellular ROL binding protein Crbp1 (RBP1) levels in VA-storing stellate cells. Reductions in organ VA signaling in obese mice correlate with increasing adiposity and fatty liver (steatosis), while with weight loss VA levels and signaling normalize. Consistent with our findings in obese mice, we show that increasing severity of fatty liver disease in humans correlates with reductions in hepatic VA, VA transcriptional signaling and Crbp1 levels in VA storing stellate cells. Thus, obesity causes a “silent” VA deficiency marked by reductions in VA levels and signaling in multiple organs, but not detected by serum VA.
- Published
- 2015
6. Explicit hypoxia targeting with tumor suppression by creating an 'obligate' anaerobic Salmonella Typhimurium strain
- Author
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Qinqin Jiang, Jian-Dong Huang, Kwok-Yung Yuen, Bin Yu, Lei Shi, Mei Yang, Xuefei Li, Yandan Yao, Lei-Han Tang, Erwei Song, Bo-Jian Zheng, and David K. Smith
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Salmonella typhimurium ,Salmonella ,Mice, Nude ,Virulence ,Breast Neoplasms ,Biology ,medicine.disease_cause ,Article ,Microbiology ,Bacteria, Anaerobic ,Mice ,Cell Line, Tumor ,Neoplasms ,medicine ,Animals ,Humans ,Hypoxia ,Multidisciplinary ,Obligate ,Hypoxia (medical) ,biology.organism_classification ,Xenograft Model Antitumor Assays ,Tumor Burden ,Biological Therapy ,Bacterial vaccine ,Essential gene ,Cell culture ,Bacterial Vaccines ,Host-Pathogen Interactions ,Female ,medicine.symptom ,Genetic Engineering ,Bacteria - Abstract
Using bacteria as therapeutic agents against solid tumors is emerging as an area of great potential in the treatment of cancer. Obligate and facultative anaerobic bacteria have been shown to infiltrate the hypoxic regions of solid tumors, thereby reducing their growth rate or causing regression. However, a major challenge for bacterial therapy of cancer with facultative anaerobes is avoiding damage to normal tissues. Consequently the virulence of bacteria must be adequately attenuated for therapeutic use. By placing an essential gene under a hypoxia conditioned promoter, Salmonella Typhimurium strain SL7207 was engineered to survive only in anaerobic conditions (strain YB1) without otherwise affecting its functions. In breast tumor bearing nude mice, YB1 grew within the tumor, retarding its growth, while being rapidly eliminated from normal tissues. YB1 provides a safe bacterial vector for anti-tumor therapies without compromising the other functions or tumor fitness of the bacterium as attenuation methods normally do.
- Published
- 2012
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